Abstract
Bacterium as a major kind of pathogen has caused a range of problems clinically, and thus became a major biological target for therapeutics. Fluorescence spectroscopy is with significant benefits for the biological sensing and detection, and in developing advanced fluorescence sensors. It is a reliable method for the semi-quantitative analysis of target analytes and provides a near unprecedented level of spatial and temporal resolution. There is thus a demand for increased specificity and new modes of detection within the field of fluorescent probes which drives research and development in the area.A discussion of the requirements for biological-eligible probes based on fluorescence technology has thus been given, and insights for designing long-wavelength/Near-Infrared probes for enhanced biological performances with reduced tissue damage are also discussed. Three research chapters follow, where fluorescence probes targeting important and essential enzymes in bacterium for tracking its function and biological processes were developed. The detection of oxidative stress among bacterium has also been reported providing a new understanding of this stimulation-induced microbiological mechanism. The new strategy to repurpose some of the existing probes for use in bacteria are finally included with both functions for the detection of enzymes and oxidative stresses.
| Date of Award | 26 Jun 2024 |
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| Original language | English |
| Awarding Institution |
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| Supervisor | Tony James (Supervisor) & Toby Jenkins (Supervisor) |