Synthesis of Novel Diagnostic systems

Abstract

In recent years, fluorescence imaging has become an indispensable tool for the exploration of biological processes, demonstrating both molecular specificity and high spatial and temporal resolution. Despite the significant progress made in this field, a number of challenges still exist which, if addressed could potentially result in the transformative development of fluorescent imaging for a plethora of biological applications. This may include the development of new fluorescent probes for the detection of unknown analytes, or the improvement of existing probes in order to enhance their properties. In this research, a fluorescent probe for the detection of hydrogen sulphide was repurposed for use as a ‘first of its kind’ fluorescent probe for the detection of hydroxylamine. In addition, the known peroxynitrite-mediated oxidation of boronic acid to phenol has been exploited for the development of a range of reaction based fluorescent probes. Initially non-fluorescent, each probe is ‘turned on’ in the presence of peroxynitrite, resulting in the formation of a highly fluorescent phenol derivatives. Such probes have been successfully evaluated during cell imaging experiments; demonstrating clear potential in the field of medical diagnostics. Specific applications may include ‘oxidative stress’, neurodegenerative disease and the evaluation of drug efficacy in the treatment of Alzheimer’s disease.

Date of Award	5 Mar 2018
Original language	English
Awarding Institution	University of Bath
Supervisor	Steven Bull (Supervisor) & Tony James (Supervisor)