Fluorescence probes serve as an attractive tools that are capable of real-time and non invasive detection of analytes in biological applications. Traditional single-responsive probes have the ability to detect one specific analyte. The introduction section includes three peer-reviewed Reviews. Representative examples of both single-responsive and dual locked probes are discussed. In addition, the development of Förster resonance energy transfer-based and two-photon probes are highlighted. The first and second data chapters discuss single-responsive probes (i.e. BHID-Bpin and DCM-Bpin) that can detect peroxynitrite with high selectivity. However, cellular environments are extremely complex, often with more than one species involved in a given biochemical process. Therefore, the remainder of the data chapters focus on the development of dual-locked fluorescent probes for reactive oxygen/nitrogen species and biothiols.
| Date of Award | 13 Dec 2021 |
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| Original language | English |
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| Awarding Institution | |
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| Supervisor | Tony James (Supervisor) & Steven Bull (Supervisor) |
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Dual-locked fluorescent probes for monitoring biological redox state: (Alternative Format Thesis)
Wu, L. (Author). 13 Dec 2021
Student thesis: Doctoral Thesis › PhD