Variations of two cell isolation methods, explant culture and enzyme dissociation were tested on adult (aged between 10-14 weeks) and foetal E15.5 (embryonic day 15.5) murine tendon tissue to extract tenocytes for subsequent cell culture. Whilst the foetal tenocytes could be extracted from the tissue with both methods, due to the inherently low cell population of adult tendon tissue, explant culture was a lengthy process for the adult tenocytes to be obtained. However, upon using 2ml blend of dispase (4mg/ml) and collagenase type IV (3mg/ml) enzymes to treat the tissue at 37 °C for 1 hour the tenocytes were released immediately from the tissue and available to culture. In addition to this, quantitative cell culture was made possible unlike explant culture, which is a qualitative technique.
|Date of Award||3 Apr 2019|
|Supervisor||Ram Sharma (Supervisor) & Marianne Ellis (Supervisor)|