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Abstract
The Hippo signaling pathway regulates organ size, tissue regeneration, and stem cell self-renewal. The two key downstream transcription coactivators in this pathway, Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), mediate the major gene regulation and biological functions of the Hippo pathway. The biological functions of YAP and TAZ in many tissues are known; however, their roles in skin wound healing remain unclear. To analyze whether YAP and/or TAZ are required for cutaneous wound healing, we performed small interfering RNA (siRNA)-mediated knockdown of YAP/TAZ in full-thickness skin wounds. YAP is strongly expressed in the nucleus and cytoplasm in the epidermis and hair follicle. Interestingly, YAP is expressed in the nucleus in the dermis at 2 and 7 days after wounding. TAZ normally localizes to the cytoplasm in the dermis but is distributed in both the nucleus and cytoplasm at 1 day after wounding. The knockdown of YAP and TAZ markedly delayed the rate of wound closure and reduced the transforming growth factor-β1 (TGF-β1) expression in the wound. YAP and TAZ also modulate the expression of TGF-β1 signaling pathway components such as Smad-2, p21, and Smad-7. These results suggest that YAP and TAZ localization to the nucleus is required for skin wound healing.
Original language | English |
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Pages (from-to) | 518-525 |
Number of pages | 8 |
Journal | Journal Of Investigative Dermatology |
Volume | 134 |
Issue number | 2 |
Early online date | 10 Oct 2013 |
DOIs | |
Publication status | Published - Feb 2014 |
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- 1 Finished
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CELLULAR AND GENETIC ANALYSIS OF LIVER DISEASE AND REGENERAT ION MODELS IN FISH
Furutani-Seiki, M.
30/03/07 → 29/03/12
Project: Research council