Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

Dielle Pierotti Procópio, Emanuele Kendrick, Rosana Goldbeck, André Ricardo de Lima Damasio, Telma Teixeira Franco, David J Leak, Yong-Su Jin, Thiago Olitta Basso

Research output: Contribution to journalReview articlepeer-review

5 Citations (SciVal)


The engineering of xylo-oligosaccharide-consuming Saccharomyces cerevisiae strains is a promising approach for more effective utilization of lignocellulosic biomass and the development of economic industrial fermentation processes. Extending the sugar consumption range without catabolite repression by including the metabolism of oligomers instead of only monomers would significantly improve second-generation ethanol production This review focuses on different aspects of the action mechanisms of xylan-degrading enzymes from bacteria and fungi, and their insertion in S. cerevisiae strains to obtain microbial cell factories able of consume these complex sugars and convert them to ethanol. Emphasis is given to different strategies for ethanol production from both extracellular and intracellular xylo-oligosaccharide utilization by S. cerevisiae strains. The suitability of S. cerevisiae for ethanol production combined with its genetic tractability indicates that it can play an important role in xylan bioconversion through the heterologous expression of xylanases from other microorganisms.

Original languageEnglish
Article number825981
JournalFrontiers in Bioengineering and Biotechnology
Publication statusPublished - 15 Feb 2022

Bibliographical note

Funding Information:
We would like to thank funding provided by FAPESP and BBSRC.

Funding Information:
This research was conducted through funding from the São Paulo Research Foundation (FAPESP), grants # 2015/50612-8, #2018/ 17172-2, #2018/01759-4, and #2019/18075-3. EK and DJL acknowledge funding from BBSRC (BB/P017460/1). Grant # 2015/50612-8 is a joint project with BB/P017460/1.

Publisher Copyright:
Copyright © 2022 Procópio, Kendrick, Goldbeck, Damasio, Franco, Leak, Jin and Basso.


  • Saccharomyces cerevisiae
  • lignocellulosic ethanol
  • xylanases
  • xylo-oligosaccharides
  • xylose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering


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