World-wide survey of an Accord insertion and its association with DDT resistance in Drosophila melanogaster

F. Catania, M. O. Kauer, P. J. Daborn, J. L. Yen, R. Ffrench-Constant, C. Schlotterer

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Abstract

Previous work showed that insecticide resistance in Drosophila melanogaster is correlated with the insertion of an Accord-like element into the 5' region of the cytochrome P450 gene, Cyp6g1. Here, we study the distribution of the Accord-like element in 673 recently collected D. melanogaster lines from 34 world-wide populations. We also examine the extent of microsatellite variability along a 180-kilobase (kb) genomic region of chromosome II encompassing the resistance gene. We confirm a 100% correlation of the Accord insertion with insecticide resistance and a significant reduction in variability extending at least 20 kb downstream of the Cyp6g1 gene. The frequency of the Accord insertion differs significantly between East African (32-55%) and nonAfrican (85-100%) populations. This pattern is consistent with a selective sweep driving the Accord insertion close to fixation in nonAfrican populations as a result of the insecticide resistance phenotype it confers. This study confirms that hitchhiking mapping can be used to identify beneficial mutations in natural populations.
Original languageEnglish
Pages (from-to)2491-2504
Number of pages14
JournalMolecular Ecology
Volume13
Issue number8
DOIs
Publication statusPublished - 2004

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    Catania, F., Kauer, M. O., Daborn, P. J., Yen, J. L., Ffrench-Constant, R., & Schlotterer, C. (2004). World-wide survey of an Accord insertion and its association with DDT resistance in Drosophila melanogaster. Molecular Ecology, 13(8), 2491-2504. https://doi.org/10.1111/j.1365-294X.2004.02263.x