Why doesn't nicotinic ACh receptor immunoreactivity knock out?

I W Jones; S Wonnacott

doi:10.1016/j.tins.2005.04.010

Why doesn't nicotinic ACh receptor immunoreactivity knock out?

I W Jones, S Wonnacott

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

62 Citations (SciVal)

Abstract

Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha 7 nicotinic ACh receptors. These data highlight the conundrum facing histologists - how 'real' is the labelling they see?

Original language	English
Pages (from-to)	343-345
Number of pages	3
Journal	Trends in Neurosciences
Volume	28
Issue number	7
DOIs	https://doi.org/10.1016/j.tins.2005.04.010
Publication status	Published - Jul 2005

Access to Document

10.1016/j.tins.2005.04.010

Cite this

@article{790f6b4e999c4efc95bddcddeb7ec7fb,

title = "Why doesn't nicotinic ACh receptor immunoreactivity knock out?",

abstract = "Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha 7 nicotinic ACh receptors. These data highlight the conundrum facing histologists - how 'real' is the labelling they see?",

author = "Jones, \{I W\} and S Wonnacott",

year = "2005",

month = jul,

doi = "10.1016/j.tins.2005.04.010",

language = "English",

volume = "28",

pages = "343--345",

journal = "Trends in Neurosciences",

issn = "0166-2236",