TY - JOUR
T1 - Why doesn't nicotinic ACh receptor immunoreactivity knock out?
AU - Jones, I W
AU - Wonnacott, S
PY - 2005/7
Y1 - 2005/7
N2 - Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha 7 nicotinic ACh receptors. These data highlight the conundrum facing histologists - how 'real' is the labelling they see?
AB - Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha 7 nicotinic ACh receptors. These data highlight the conundrum facing histologists - how 'real' is the labelling they see?
UR - http://dx.doi.org/10.1016/j.tins.2005.04.010
U2 - 10.1016/j.tins.2005.04.010
DO - 10.1016/j.tins.2005.04.010
M3 - Article
SN - 0166-2236
VL - 28
SP - 343
EP - 345
JO - Trends in Neurosciences
JF - Trends in Neurosciences
IS - 7
ER -