What has single-cell transcriptomics taught us about long non-coding RNAs in the ventricular-subventricular zone?

Jemima Becker, Bin Sun, Farah Alammari, Wilfried Haerty, Keith W. Vance, Francis George Szele

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)


Long non-coding RNA (lncRNA) function is mediated by the process of transcription or through transcript-dependent associations with proteins or nucleic acids to control gene regulatory networks. Many lncRNAs are transcribed in the ventricular-subventricular zone (V-SVZ), a postnatal neural stem cell niche. lncRNAs in the V-SVZ are implicated in neurodevelopmental disorders, cancer, and brain disease, but their functions are poorly understood. V-SVZ neurogenesis capacity declines with age due to stem cell depletion and resistance to neural stem cell activation. Here we analyzed V-SVZ transcriptomics by pooling current single-cell RNA-seq data. They showed consistent lncRNA expression during stem cell activation, lineage progression, and aging. In conjunction with epigenetic and genetic data, we predicted V-SVZ lncRNAs that regulate stem cell activation and differentiation. Some of the lncRNAs validate known epigenetic mechanisms, but most remain uninvestigated. Our analysis points to several lncRNAs that likely participate in key aspects of V-SVZ stem cell activation and neurogenesis in health and disease.

Original languageEnglish
Pages (from-to)354-376
Number of pages23
JournalStem Cell Reports
Issue number1
Early online date15 Dec 2022
Publication statusPublished - 10 Jan 2023

Bibliographical note

Funding Information:
W.H. was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Core Strategic Programme Grant BB/CSP1720/1 and its constituent work packages (BBS/E/T/000PR9818 and BBS/E/T/000PR9819).


  • adult neurogenesis
  • epigenetics
  • lncRNA
  • neural stem cells
  • scRNA-seq
  • V-SVZ

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology


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