Visualization of topical drug delivery with label-free chemical imaging

Natalie A. Belsey; Vasundhara Tyagi; Jean Luc Vorng; Alex Dexter; Dimitrios Tsikritsis; Panagiota Zarmpi; Richard H. Guy

doi:10.1117/12.3008776

Visualization of topical drug delivery with label-free chemical imaging

Natalie A. Belsey, Vasundhara Tyagi, Jean Luc Vorng, Alex Dexter, Dimitrios Tsikritsis, Panagiota Zarmpi, Richard H. Guy

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Abstract

Recent advances in label-free chemical imaging approaches have yielded new methods for measuring drug distribution in biological tissues. Such techniques include Raman spectroscopy and multiphoton microscopies, in addition to a suite of powerful mass spectrometry imaging methods. These techniques offer complementary information, with different strengths and limitations. By combining several of these datasets using image registration, powerful visualization can be achieved, combining high chemical specificity and sensitivity with sub-micron spatial resolution. Here we present a correlative imaging example that combines nonlinear optical spectroscopies and secondary ion mass spectroscopy imaging, applied to the same skin sample, for label-free visualization of the tissue structure and the distribution of an applied model compound.

Original language	English
Title of host publication	Visualizing and Quantifying Drug Distribution in Tissue VIII
Editors	Kin Foong Chan, Conor L. Evans
Place of Publication	Washington, U. S. A.
Publisher	SPIE
ISBN (Electronic)	9781510669017
DOIs	https://doi.org/10.1117/12.3008776
Publication status	Published - 12 Mar 2024
Event	Visualizing and Quantifying Drug Distribution in Tissue VIII 2024 - San Francisco, USA United States Duration: 27 Jan 2024 → …

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12821
ISSN (Print)	1605-7422

Conference

Conference	Visualizing and Quantifying Drug Distribution in Tissue VIII 2024
Country/Territory	USA United States
City	San Francisco
Period	27/01/24 → …

Funding

This work was funded by the Food and Drug Administration (FDA) of the U.S. Department of Health and Human Services (HHS) as part of a financial assistance award (1-U01-FD006533). The contents of this article are those of the authors and do not necessarily represent the official views of, nor an endorsement by, FDA/HHS or the U.S. Government. The development of the methodologies used in this work was funded in part by Haleon CH SARL, and the authors are grateful to Dr Mila Boncheva Bettex for helpful discussions. Methodologies were also funded in part by the UK Department for Science, Innovation and Technology through the National Metrology Programme, and the authors thank Prof. Ian Gilmore (NPL) for helpful discussions. Support by the Community for Analytical Measurement Science is also gratefully acknowledged for a 2020 CAMS Fellowship Award to Natalie Belsey, funded by the Analytical Chemistry Trust Fund.

Funders	Funder number
U.S. Department of Health and Human Services	1-U01-FD006533

Keywords

Correlative imaging
mass spectrometry imaging
SIMS
skin
SRS microscopy
topical drug delivery

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.3008776

Cite this

Belsey, N. A., Tyagi, V., Vorng, J. L., Dexter, A., Tsikritsis, D., Zarmpi, P., & Guy, R. H. (2024). Visualization of topical drug delivery with label-free chemical imaging. In K. F. Chan, & C. L. Evans (Eds.), Visualizing and Quantifying Drug Distribution in Tissue VIII Article 1282107 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12821). SPIE. https://doi.org/10.1117/12.3008776

Visualization of topical drug delivery with label-free chemical imaging. / Belsey, Natalie A.; Tyagi, Vasundhara; Vorng, Jean Luc et al.
Visualizing and Quantifying Drug Distribution in Tissue VIII. ed. / Kin Foong Chan; Conor L. Evans. Washington, U. S. A.: SPIE, 2024. 1282107 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12821).

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Belsey, NA, Tyagi, V, Vorng, JL, Dexter, A, Tsikritsis, D, Zarmpi, P & Guy, RH 2024, Visualization of topical drug delivery with label-free chemical imaging. in KF Chan & CL Evans (eds), Visualizing and Quantifying Drug Distribution in Tissue VIII., 1282107, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12821, SPIE, Washington, U. S. A., Visualizing and Quantifying Drug Distribution in Tissue VIII 2024, San Francisco, USA United States, 27/01/24. https://doi.org/10.1117/12.3008776

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Visualization of topical drug delivery with label-free chemical imaging

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