Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

Lu Li; Lei Feng; Ming Zhang; Xin He; Shengqiao Luan; Chao Wang; Tony D. James; Houli Zhang; Huilian Huang; Xiaochi Ma

doi:10.1039/d0cc00197j

Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

Lu Li, Lei Feng, Ming Zhang, Xin He, Shengqiao Luan, Chao Wang, Tony D. James, Houli Zhang, Huilian Huang, Xiaochi Ma

Research output: Contribution to journal › Article › peer-review

18 Citations (SciVal)

Abstract

A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.

Original language	English
Pages (from-to)	4640-4643
Number of pages	4
Journal	Chemical communications (Cambridge, England)
Volume	56
Issue number	34
DOIs	https://doi.org/10.1039/d0cc00197j
Publication status	Published - 24 Apr 2020

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc00197jLicence: CC BY

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title = "Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe",

abstract = "A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.",

author = "Lu Li and Lei Feng and Ming Zhang and Xin He and Shengqiao Luan and Chao Wang and James, \{Tony D.\} and Houli Zhang and Huilian Huang and Xiaochi Ma",

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doi = "10.1039/d0cc00197j",

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T1 - Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

AU - Li, Lu

AU - Feng, Lei

AU - Zhang, Ming

AU - He, Xin

AU - Luan, Shengqiao

AU - Wang, Chao

AU - James, Tony D.

AU - Zhang, Houli

AU - Huang, Huilian

AU - Ma, Xiaochi

PY - 2020/4/24

Y1 - 2020/4/24

N2 - A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.

AB - A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.

UR - https://www.scopus.com/pages/publications/85084167153

U2 - 10.1039/d0cc00197j

DO - 10.1039/d0cc00197j

M3 - Article

C2 - 32270142

AN - SCOPUS:85084167153

SN - 1359-7345

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SP - 4640

EP - 4643

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

IS - 34

ER -

Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

Abstract

ASJC Scopus subject areas

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