Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe

Lei Feng, Ying Deng, Shufan Song, Yanqiu Sun, Jingnan Cui, Xiaochi Ma, Lingling Jin, Yan Wang, Tony D. James, Chao Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Lipase found in the gut microbiota participates in the digestion and absorption of dietary fats. As such, the gut microbiota is involved in the regulation of the host metabolism, affecting the levels of lipids and free fatty acids, ultimately resulting in obesity. In this study, an enzymatic activatable near-infrared fluorescent probe, DDAO-C6, was developed for visually sensing endogenous lipase from gut microbes. Using DDAO-C6, a cultivated intestinal yeast strain was rapidly identified from human feces that exhibited high lipase expression and was identified as Trichosporon asahii Y2. We then determined that the colonization of the gut of mice with T. asahii Y2 increased lipase activity in the digestive tract and promoted obesity and hyperlipidemia when the mice were fed high fat diets. Above all, the present research resulted in a fluorescence visualization tool for the functional investigation of gut microbiota associated with obesity and disorders of lipid metabolism.

Original languageEnglish
Pages (from-to)11216-11223
Number of pages8
JournalAnalytical Chemistry
Volume94
Issue number32
DOIs
Publication statusPublished - 16 Aug 2022

ASJC Scopus subject areas

  • Analytical Chemistry

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