Visual identification of gut bacteria and determination of natural inhibitors using a fluorescent probe selective for PGP-1

Chao Wang, Zhenhao Tian, Ming Zhang, Ying Deng, Xiangge Tian, Lei Feng, Jingnan Cui, Tony D. James, Xiaochi Ma

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)

Abstract

PGP-1 is a bacterial hydrolase that can hydrolyze the amide bond of the L-pyroglutamate (L-pGlu) residue at the amino terminus of proteins and peptides. Guided by the biological function of PGP-1, an off-on NIR fluorescent probe DDPA was developed for the visual sensing of PGP-1 by conjugating pyroglutamic acid (recognition group) and DDAN (fluorophore). Using intestinal bacteria cultivation, eight bacteria strains with active PGP-1 were identified and cultivated efficiently using DDPA. In addition, three natural inhibitors against PGP-1 were isolated from the medical herb Psoralea corylifolia, which could be used to interfere with bacterial metabolism in the gut. As such, the fluorescent probe DDPA provides an efficient method and potential tool for the investigation of intestinal microbiota.

Original languageEnglish
Article number339280
JournalAnalytica Chimica Acta
Volume1191
Early online date15 Nov 2021
DOIs
Publication statusPublished - 25 Jan 2022

Keywords

  • Fluorescent probe
  • High throughput screening
  • Intestinal bacteria
  • Near-infrared
  • PGP-1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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