Versatile Ratiometric Fluorescent Probe Based on the Two-Isophorone Fluorophore for Sensing Nitroxyl

Huan Li, Chengcheng Wang, Lei Cai, Xiang Yu, Luling Wu, Nannan Yuan, Yiming Zhu, Nengqin Jia, Tony D. James, Chusen Huang

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Abstract

Nitroxyl (HNO) is closely linked with numerous biological processes. Fluorescent probes provide a visual tool for determining HNO. Due to fluorescence quenching by HNO-responsive recognition groups, most of the current fluorescent probes exhibit an "off-on"fluorescence response. As such, the single fluorescence signal of these probes is easily affected by external factors such as the microenvironment, sensor concentration, and photobleaching. Herein, we have developed a ratiometric fluorescent probe (CHT-P) based on our previously developed two-isophorone fluorophore. CHT-P could be used to determine HNO through ratiometric signal readouts with high selectivity and sensitivity, ensuring the accurate quantitative detection of HNO. Additionally, the probe exhibited low cytotoxicity, was cell permeable, and could be used for ratiometric imaging of HNO in cells. Finally, CHT-P-coated portable test strips were used to determine HNO using the solid-state fluorescence signal readout.

Original languageEnglish
Pages (from-to)15913-15920
JournalIndustrial and Engineering Chemistry Research
Volume60
Issue number44
Early online date26 Oct 2021
DOIs
Publication statusPublished - 10 Nov 2021

Bibliographical note

Funding Information:
L.W. wishes to thank the China Scholarship Council and the University of Bath for supporting his PhD in the UK. T.D.J. wishes to thank the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University, for support (2020ZD01). C.H. thanks the National Natural Science Foundation of China (Grants 21672150, 21302125), the Alexander von Humboldt Foundation (AvH), the Shanghai Rising-Star Program (19QA1406400), the Doctoral Fund of Ministry of Education of China (grant no. 20133127120005), the Shanghai Government (18DZ2254200), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT_16R49).

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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