Using false discovery rates to benchmark SNP-callers in next-generation sequencing projects

Rhys A Farrer, Daniel A Henk, Dan MacLean, David J Studholme, Matthew C Fisher

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Sequence alignments form the basis for many comparative and population genomic studies. Alignment
tools provide a range of accuracies dependent on the divergence between the sequences and the alignment
methods. Despite widespread use, there is no standard method for assessing the accuracy of a dataset and
alignment strategy after resequencing. We present a framework and tool for determining the overall
accuracies of an input read dataset, alignment and SNP-calling method providing an isolate in that dataset
has a corresponding, or closely related reference sequence available. In addition to this tool for comparing
from an alignment using binomial probabilities for an expected error rate. We benchmark this method
against other SNP callers using our FDR method with three fungal genomes, finding that it was able achieve
a high level of accuracy. These tools are available at http://cfdr.sourc
Original languageEnglish
Article number1512
JournalScientific Reports
Publication statusPublished - 21 Mar 2013


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