Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S)

F A Sattar, Daniel J Darley, F Politano, Timothy J Woodman, Michael D Threadgill, Matthew D Lloyd

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

alpha-Methylacyl-CoA racemase (AMACR;double dagger P504S) catalysed exchange of straight-chain fatty acyl-CoA alpha-protons. One alpha-proton was removed in each catalytic cycle, with the pro-S proton preferred. This reaction was most efficient for straight-chain substrates with longer side-chains. 2-Methyldecanoyl-CoA underwent alpha-proton exchange 3x more efficiently (as judged by K-cat/K-m) than decanoyl-CoA.
LanguageEnglish
Pages3348-3350
Number of pages3
JournalChemical Communications
Volume46
Issue number19
Early online date29 Mar 2010
DOIs
StatusPublished - 21 May 2010

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Acyl Coenzyme A
Protons
Ion exchange
Coenzyme A
alpha-methylacyl-CoA racemase
Substrates

Cite this

Unexpected stereoselective exchange of straight-chain fatty acyl-CoA α-protons by human α-methylacyl-CoA racemase 1A (P504S). / Sattar, F A; Darley, Daniel J; Politano, F; Woodman, Timothy J; Threadgill, Michael D; Lloyd, Matthew D.

In: Chemical Communications, Vol. 46, No. 19, 21.05.2010, p. 3348-3350.

Research output: Contribution to journalArticle

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