Ultrastructural localisation of neuronal nicotinic acetylcholine receptor alpha4 subunit in rat nigro-striatal neurons

A L Bradly, I W Jones, Susan Wonnacott

Research output: Contribution to journalArticle

Abstract

Somato-dendritic and presynaptic nicotinic acetylcholine receptors (nAChR) modulate dopamine release from nigro-striatal neurons. There is pharmacological evidence for α4* and α3* nAChR subtypes at striatal dopaminergic terminals, but the distribution and localisation of these subunits remains unclear. Questions such as the co-localisation or segregation of α3* and α4* subunits in the same or different neuronal populations, respectively, can be answered by analysing the distribution of subunits at the ultrastructural level.
As a first step, the α4 nAChR subunit has been localised by immunocytochemistry using antibodies targeted to both intracellular and extracellular epitopes. Tyrosine hydroxylase (TH) immunoreactivity was examined in parallel to identify dopaminergic neurons. At the light and confocal microscopy level, α4 and TH immunoreactivities co-localised in cell bodies of nigro-striatal dopaminergic neurons and in striatal synaptosomes. The subcellular distribution of the α4 subunit in relation to the local neurochemical environment has been investigated, using immuno-electron microscopy techniques. Double pre-embedding immuno-labelling, using diaminobenzidine to visualise TH and gold particles for the α4, confirmed the presynaptic location of the α4 nAChR subunit in TH positive terminals, supporting the pharmacological data. TH positive nigral cell bodies and proximal dendrites were also densely labelled for the α4 subunit. These techniques facilitate analysis of the comparative distribution of α4 and α3 nAChR subunits within nigrostriatal neurons.
LanguageEnglish
Article number374.6
Pages980
Number of pages1
JournalSociety for Neuroscience Abstracts
Volume27
Issue number1
StatusPublished - 2001
Event31st Annual Meeting of the Society for Neuroscience - San Diego, USA United States
Duration: 10 Nov 200115 Nov 2001

Fingerprint

Corpus Striatum
Nicotinic Receptors
Tyrosine 3-Monooxygenase
Neurons
Dopaminergic Neurons
Pharmacology
Immunoelectron Microscopy
Synaptosomes
Substantia Nigra
Dendrites
Confocal Microscopy
Gold
Epitopes
Dopamine
Immunohistochemistry
nicotinic acetylcholine receptor alpha4 subunit
Light
Antibodies
Population

Cite this

Ultrastructural localisation of neuronal nicotinic acetylcholine receptor alpha4 subunit in rat nigro-striatal neurons. / Bradly, A L; Jones, I W; Wonnacott, Susan.

In: Society for Neuroscience Abstracts, Vol. 27, No. 1, 374.6, 2001, p. 980.

Research output: Contribution to journalArticle

@article{10540b964d604b17b4a7a6348f3d08f6,
title = "Ultrastructural localisation of neuronal nicotinic acetylcholine receptor alpha4 subunit in rat nigro-striatal neurons",
abstract = "Somato-dendritic and presynaptic nicotinic acetylcholine receptors (nAChR) modulate dopamine release from nigro-striatal neurons. There is pharmacological evidence for α4* and α3* nAChR subtypes at striatal dopaminergic terminals, but the distribution and localisation of these subunits remains unclear. Questions such as the co-localisation or segregation of α3* and α4* subunits in the same or different neuronal populations, respectively, can be answered by analysing the distribution of subunits at the ultrastructural level.As a first step, the α4 nAChR subunit has been localised by immunocytochemistry using antibodies targeted to both intracellular and extracellular epitopes. Tyrosine hydroxylase (TH) immunoreactivity was examined in parallel to identify dopaminergic neurons. At the light and confocal microscopy level, α4 and TH immunoreactivities co-localised in cell bodies of nigro-striatal dopaminergic neurons and in striatal synaptosomes. The subcellular distribution of the α4 subunit in relation to the local neurochemical environment has been investigated, using immuno-electron microscopy techniques. Double pre-embedding immuno-labelling, using diaminobenzidine to visualise TH and gold particles for the α4, confirmed the presynaptic location of the α4 nAChR subunit in TH positive terminals, supporting the pharmacological data. TH positive nigral cell bodies and proximal dendrites were also densely labelled for the α4 subunit. These techniques facilitate analysis of the comparative distribution of α4 and α3 nAChR subunits within nigrostriatal neurons.",
author = "Bradly, {A L} and Jones, {I W} and Susan Wonnacott",
year = "2001",
language = "English",
volume = "27",
pages = "980",
journal = "Society for Neuroscience Abstracts",
issn = "0190-5295",
number = "1",

}

TY - JOUR

T1 - Ultrastructural localisation of neuronal nicotinic acetylcholine receptor alpha4 subunit in rat nigro-striatal neurons

AU - Bradly, A L

AU - Jones, I W

AU - Wonnacott, Susan

PY - 2001

Y1 - 2001

N2 - Somato-dendritic and presynaptic nicotinic acetylcholine receptors (nAChR) modulate dopamine release from nigro-striatal neurons. There is pharmacological evidence for α4* and α3* nAChR subtypes at striatal dopaminergic terminals, but the distribution and localisation of these subunits remains unclear. Questions such as the co-localisation or segregation of α3* and α4* subunits in the same or different neuronal populations, respectively, can be answered by analysing the distribution of subunits at the ultrastructural level.As a first step, the α4 nAChR subunit has been localised by immunocytochemistry using antibodies targeted to both intracellular and extracellular epitopes. Tyrosine hydroxylase (TH) immunoreactivity was examined in parallel to identify dopaminergic neurons. At the light and confocal microscopy level, α4 and TH immunoreactivities co-localised in cell bodies of nigro-striatal dopaminergic neurons and in striatal synaptosomes. The subcellular distribution of the α4 subunit in relation to the local neurochemical environment has been investigated, using immuno-electron microscopy techniques. Double pre-embedding immuno-labelling, using diaminobenzidine to visualise TH and gold particles for the α4, confirmed the presynaptic location of the α4 nAChR subunit in TH positive terminals, supporting the pharmacological data. TH positive nigral cell bodies and proximal dendrites were also densely labelled for the α4 subunit. These techniques facilitate analysis of the comparative distribution of α4 and α3 nAChR subunits within nigrostriatal neurons.

AB - Somato-dendritic and presynaptic nicotinic acetylcholine receptors (nAChR) modulate dopamine release from nigro-striatal neurons. There is pharmacological evidence for α4* and α3* nAChR subtypes at striatal dopaminergic terminals, but the distribution and localisation of these subunits remains unclear. Questions such as the co-localisation or segregation of α3* and α4* subunits in the same or different neuronal populations, respectively, can be answered by analysing the distribution of subunits at the ultrastructural level.As a first step, the α4 nAChR subunit has been localised by immunocytochemistry using antibodies targeted to both intracellular and extracellular epitopes. Tyrosine hydroxylase (TH) immunoreactivity was examined in parallel to identify dopaminergic neurons. At the light and confocal microscopy level, α4 and TH immunoreactivities co-localised in cell bodies of nigro-striatal dopaminergic neurons and in striatal synaptosomes. The subcellular distribution of the α4 subunit in relation to the local neurochemical environment has been investigated, using immuno-electron microscopy techniques. Double pre-embedding immuno-labelling, using diaminobenzidine to visualise TH and gold particles for the α4, confirmed the presynaptic location of the α4 nAChR subunit in TH positive terminals, supporting the pharmacological data. TH positive nigral cell bodies and proximal dendrites were also densely labelled for the α4 subunit. These techniques facilitate analysis of the comparative distribution of α4 and α3 nAChR subunits within nigrostriatal neurons.

UR - http://www.sfn.org/index.aspx?pagename=abstracts_ampublications

M3 - Article

VL - 27

SP - 980

JO - Society for Neuroscience Abstracts

T2 - Society for Neuroscience Abstracts

JF - Society for Neuroscience Abstracts

SN - 0190-5295

IS - 1

M1 - 374.6

ER -