Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG

Sanjay Timilsina; Helena De Puig; Pawan Jolly; Josh Rainbow; Nolan Durr; Pedro Estrela; James Collins; Donald E. Ingber

Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG

Sanjay Timilsina, Helena De Puig, Pawan Jolly, Josh Rainbow, Nolan Durr, Pedro Estrela, James Collins, Donald E. Ingber

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Abstract

The COVID-19 pandemic has demonstrated the need for better understanding of the kinetics of anti-SARSCoV-2 antibody production and development of serological assays for multiple viral antigens. Electrochemical (EC) sensor platforms offer the potential to develop rapid, sensitive, point-of-care (POC) diagnostics for this type of application. Here, we describe multiplexed EC biosensors with novel antifouling properties that detect anti-SARSCoV-2 immunoglobulin G (IgG) against spike protein (S), spike receptor-binding domain (RBD), and nucleocapsid (NC) antigens. This POC assay was validated using 69 clinical blood samples and obtained 96% sensitivity and 100% specificity with area under the curve (AUC) of 0.98 for multiplexed detection of anti-SARS-CoV-2 IgG.

Original language	English
Title of host publication	Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1487-1488
ISBN (Electronic)	978-173341903-1
Publication status	Published - 10 Oct 2021
Event	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Duration: 10 Oct 2021 → 14 Oct 2021

Conference

Conference	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Period	10/10/21 → 14/10/21

Cite this

Timilsina, S., De Puig, H., Jolly, P., Rainbow, J., Durr, N., Estrela, P., Collins, J., & Ingber, D. E. (2021). Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG. In Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1487-1488). Chemical and Biological Microsystems Society.

Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG. / Timilsina, Sanjay; De Puig, Helena; Jolly, Pawan et al.
Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1487-1488.

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Timilsina, S, De Puig, H, Jolly, P, Rainbow, J, Durr, N, Estrela, P, Collins, J & Ingber, DE 2021, Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG. in Proceedings of MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1487-1488, MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 10/10/21.

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title = "Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG",

abstract = "The COVID-19 pandemic has demonstrated the need for better understanding of the kinetics of anti-SARSCoV-2 antibody production and development of serological assays for multiple viral antigens. Electrochemical (EC) sensor platforms offer the potential to develop rapid, sensitive, point-of-care (POC) diagnostics for this type of application. Here, we describe multiplexed EC biosensors with novel antifouling properties that detect anti-SARSCoV-2 immunoglobulin G (IgG) against spike protein (S), spike receptor-binding domain (RBD), and nucleocapsid (NC) antigens. This POC assay was validated using 69 clinical blood samples and obtained 96% sensitivity and 100% specificity with area under the curve (AUC) of 0.98 for multiplexed detection of anti-SARS-CoV-2 IgG. ",

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AU - Timilsina, Sanjay

AU - De Puig, Helena

AU - Jolly, Pawan

AU - Rainbow, Josh

AU - Durr, Nolan

AU - Estrela, Pedro

AU - Collins, James

AU - Ingber, Donald E.

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AB - The COVID-19 pandemic has demonstrated the need for better understanding of the kinetics of anti-SARSCoV-2 antibody production and development of serological assays for multiple viral antigens. Electrochemical (EC) sensor platforms offer the potential to develop rapid, sensitive, point-of-care (POC) diagnostics for this type of application. Here, we describe multiplexed EC biosensors with novel antifouling properties that detect anti-SARSCoV-2 immunoglobulin G (IgG) against spike protein (S), spike receptor-binding domain (RBD), and nucleocapsid (NC) antigens. This POC assay was validated using 69 clinical blood samples and obtained 96% sensitivity and 100% specificity with area under the curve (AUC) of 0.98 for multiplexed detection of anti-SARS-CoV-2 IgG.

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Ultrasensitive electrochemical sensor platform for multiplexed detection of multiple anti-SARS-CoV-2 IgG

Abstract

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