TY - JOUR
T1 - Ultrasensitive detection based on an aptamer beacon electron transfer chain
AU - Mir, Monica
AU - Jenkins, A Toby A
AU - Katakis, Ioannis
PY - 2008/10
Y1 - 2008/10
N2 - The construction of an ultrasensitive, reagentless, target label free electrochemical aptamer sensor (aptasensor) for thrombin detection is described. The aptasensor is based on a chronoamperometric beacon system for biomolecular recognition. The ferrocene-labeled aptamer adopts a 3-D conformational change when interacts with thrombin. Thus the ferrocene label is approached to the microperoxise-11 (MP-11) attached on the electrode surface. The thrombin–aptamer interaction is detected via a microperoxidase mediated electron transfer between the ferrocene and the surface. This system was demonstrated with surface plasmon resonance, impedance spectroscopy and chronoamperometry measurements, obtaining higher sensitivity (30 fM) with impedance spectroscopy.
AB - The construction of an ultrasensitive, reagentless, target label free electrochemical aptamer sensor (aptasensor) for thrombin detection is described. The aptasensor is based on a chronoamperometric beacon system for biomolecular recognition. The ferrocene-labeled aptamer adopts a 3-D conformational change when interacts with thrombin. Thus the ferrocene label is approached to the microperoxise-11 (MP-11) attached on the electrode surface. The thrombin–aptamer interaction is detected via a microperoxidase mediated electron transfer between the ferrocene and the surface. This system was demonstrated with surface plasmon resonance, impedance spectroscopy and chronoamperometry measurements, obtaining higher sensitivity (30 fM) with impedance spectroscopy.
UR - http://www.scopus.com/inward/record.url?scp=52349083639&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.elecom.2008.04.040
U2 - 10.1016/j.elecom.2008.04.040
DO - 10.1016/j.elecom.2008.04.040
M3 - Article
SN - 1388-2481
VL - 10
SP - 1533
EP - 1536
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 10
ER -