Ultrasensitive detection based on an aptamer beacon electron transfer chain

Monica Mir, A Toby A Jenkins, Ioannis Katakis

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The construction of an ultrasensitive, reagentless, target label free electrochemical aptamer sensor (aptasensor) for thrombin detection is described. The aptasensor is based on a chronoamperometric beacon system for biomolecular recognition. The ferrocene-labeled aptamer adopts a 3-D conformational change when interacts with thrombin. Thus the ferrocene label is approached to the microperoxise-11 (MP-11) attached on the electrode surface. The thrombin–aptamer interaction is detected via a microperoxidase mediated electron transfer between the ferrocene and the surface. This system was demonstrated with surface plasmon resonance, impedance spectroscopy and chronoamperometry measurements, obtaining higher sensitivity (30 fM) with impedance spectroscopy.
Original languageEnglish
Pages (from-to)1533-1536
Number of pages4
JournalElectrochemistry Communications
Volume10
Issue number10
Early online date15 Jul 2008
DOIs
Publication statusPublished - Oct 2008

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