Abstract
Through mechanistic work and rational design, we have developed the fastest organometallic abiotic Cys bioconjugation. As a result, the developed organometallic Au(III) bioconjugation reagents enable selective labeling of Cys moieties down to picomolar concentrations and allow for the rapid construction of complex heterostructures from peptides, proteins, and oligonucleotides. This work showcases how organometallic chemistry can be interfaced with biomolecules and lead to a range of reactivities that are largely unmatched by classical organic chemistry tools.
Original language | English |
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Pages (from-to) | 12365-12374 |
Journal | Journal of the American Chemical Society |
Volume | 146 |
Issue number | 18 |
Early online date | 24 Apr 2024 |
DOIs | |
Publication status | Published - 8 May 2024 |
Funding
A.M.S. thanks NIGMS (R35GM124746). Portions of this work were supported by University of Bath and EPSRC (to C.G.F.), NSF CHE-2003946 and NSF CHE-2153972 (to K.N.H.), NSF CHE-2003946 (to H.D.M.), and Noble Family Innovation Fund administered by UCLA California Nanosystems Institute (CNSI) (to A. M. S.). J.A.L. and E.J.O. thank NIGMS for their corresponding support: R35GM145286 and T32GM145388. This work used computational and storage services associated with the Hoffman2 Shared Cluster provided by the UCLA Institute for Digital Research and Education\u2019s Research Technology Group. The authors are grateful to Dr. Martin Phillips for his assistance with the stopped-flow experiments.
Funders | Funder number |
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California NanoSystems Institute | |
University of Bath | |
Noble Family Innovation Fund | |
Engineering and Physical Sciences Research Council | |
National Science Foundation | CHE-2003946, CHE-2153972 |
National Science Foundation |
ASJC Scopus subject areas
- Catalysis
- General Chemistry
- Biochemistry
- Colloid and Surface Chemistry