In light of widespread resurgence of the respiratory disease whooping cough, ongoing research aims to identify changes to the causative bacterium, Bordetella pertussis. B. pertussis is traditionally described as a highly clonal species at the single-base level, hence our research largely focusses on identifying differences between strains on a whole-genome scale. Long-read sequencing has enabled us to produce closed genome sequences for B. pertussis isolates on an unprecedented scale, allowing visualisation of extensive inter-strain genomic rearrangements. This work also led to the unexpected discovery of a second phenomenon: large duplications which are present in some recent isolates but not in the B. pertussis reference genome. Intriguingly, these duplications may be present in only a fraction of the cells of duplication-carrying strains. At London Calling, I will discuss this developing story, including the essential role of long and ultra-long nanopore sequencing in proving the existence of the duplications and characterising variable populations, alongside continuing work to quantify the phenotypic effects of the duplications.
|Publication status||Published - 24 May 2019|
|Event||London Calling: Oxford Nanopore Technologies Annual Meeting - Old Billingsgate, London, UK United Kingdom|
Duration: 22 May 2019 → 24 May 2019
|Conference||London Calling: Oxford Nanopore Technologies Annual Meeting|
|Country||UK United Kingdom|
|Period||22/05/19 → 24/05/19|
Ring, N. (2019). Ultra-long reads and ultra-long duplications: Deciphering the mysteries of the Bordetella pertussis genome. Paper presented at London Calling: Oxford Nanopore Technologies Annual Meeting, London, UK United Kingdom. https://vimeo.com/338855148