TY - JOUR
T1 - Tyrosine kinase regulation of nuclear envelope assembly
AU - Byrne, Richard D.
AU - Larijani, Banafshé
AU - Poccia, Dominic L.
N1 - Funding Information:
We are very grateful to Paul Davies of Macrae & Company for sea urchin procurement, Kathy Foltz for the kind gift of the AmPLCγpep and SFK1/7 antisera, Mike Mitchell (CR-UK, Bioinformatics & Biostatistics) for sea urchin genome analysis and Jackie Eastman for assistance with confocal microscopy. This work was supported by CR-UK and an Amherst College Faculty Research Award from the H. Axel Schupf '57 Fund for Intellectual Life (DLP). RDB was funded by an Amherst College Copeland Fellowship and CR-UK.
PY - 2009/1/20
Y1 - 2009/1/20
N2 - We have described the co-localization of PLCγ and SFK1/7 in vivo and in vitro, and demonstrated their recruitment together to nuclei during membrane binding. We hypothesize that SFK1/7 is activated in response to GTP hydrolysis, phosphorylating and priming PLCγ for activation and thus producing the fusogen DAG during NE assembly. In addition to enhancing our understanding of the temporal and spatial mechanisms of NE formation, this pathway offers a novel link between protein regulation and lipid metabolism that has implications for general membrane fusion events.
AB - We have described the co-localization of PLCγ and SFK1/7 in vivo and in vitro, and demonstrated their recruitment together to nuclei during membrane binding. We hypothesize that SFK1/7 is activated in response to GTP hydrolysis, phosphorylating and priming PLCγ for activation and thus producing the fusogen DAG during NE assembly. In addition to enhancing our understanding of the temporal and spatial mechanisms of NE formation, this pathway offers a novel link between protein regulation and lipid metabolism that has implications for general membrane fusion events.
UR - http://www.scopus.com/inward/record.url?scp=66449107842&partnerID=8YFLogxK
U2 - 10.1016/j.advenzreg.2008.12.010
DO - 10.1016/j.advenzreg.2008.12.010
M3 - Article
C2 - 19344633
AN - SCOPUS:66449107842
SN - 0065-2571
VL - 49
SP - 148
EP - 156
JO - Advances in Enzyme Regulation
JF - Advances in Enzyme Regulation
IS - 1
ER -
