Two-photon small-molecule fluorescence-based agents for sensing, imaging, and therapy within biological systems

Luling Wu, Jihong Liu, Ping Li, Bo Tang, Tony D. James

Research output: Contribution to journalReview articlepeer-review


In this tutorial review, we will explore recent advances for the design, construction and application of two-photon excited fluorescence (TPEF)-based small-molecule probes. The advantages of TPEF-based probes include deep tissue penetration and minimal photo-damage. We discuss the underlying two-photon (TP) fluorophores including hemicyanine and design strategies such as Forster resonance energy transfer (FRET). Moreover, we emphasize applications for the detection or imaging of cations, anions, small neutral molecules, biomacromolecules, cellular microenvironments, subcellular localization and dual-responsive systems. Examples of photodynamic therapy (PDT) using TP irradiation are also illustrated.

Original languageEnglish
Pages (from-to)702-734
Number of pages33
JournalChemical Society Reviews
Issue number2
Publication statusPublished - 21 Jan 2021

ASJC Scopus subject areas

  • Chemistry(all)

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