“Turn-on” and ratiometric detection of carboxylesterases using acetyl-based fluorescent strategies for bacterial applications

Kai Cheng Yan; Fang Fang Guo; Ling Zhang; Naing Thet; Adam C. Sedgwick; Yuan Wang; Zhi Hong Xu; Tony D. James; A. Toby A. Jenkins

doi:10.1016/j.snb.2023.135121

“Turn-on” and ratiometric detection of carboxylesterases using acetyl-based fluorescent strategies for bacterial applications

Kai Cheng Yan, Fang Fang Guo, Ling Zhang, Naing Thet, Adam C. Sedgwick, Yuan Wang, Zhi Hong Xu, Tony D. James, A. Toby A. Jenkins

Research output: Contribution to journal › Article › peer-review

2 Citations (SciVal)

Abstract

Carboxylesterases (CEs) are involved in the biotransformation of numerous drugs, environmental toxicants, and carcinogens containing ester groups. Abnormal CEs levels are associated with various diseases, and as such they are widely studied in cells. CEs are also characteristic biomarkers for assisting in the detection of certain strains of bacteria and are involved in important biological processes. Herein, we used two probes including an indene-chalcone-based fluorescent probe which displays green “turn-on” fluorescence response, and a merocyanine-based ratiometric probe to detect CEs, as well as detect and image S. aureus and their chronic biofilms using acetate ester as recognition group.

Original language	English
Article number	135121
Journal	Sensors and Actuators B: Chemical
Volume	404
Early online date	12 Dec 2023
DOIs	https://doi.org/10.1016/j.snb.2023.135121
Publication status	Published - 1 Apr 2024

Funding

ATAJ and NT would like to acknowledge funding from the EPSRC , grant reference: EP/V00462X/1 . TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University ( 2020ZD01 ) for support. ACS would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support. YW thanks the National Natural Science Foundation of China ( 21907023, 21001040 ), Henan Province Science and Technology Project ( 232102320092 ) for support. ZHX thanks the Joint Program for Fostering Talents of National Natural Science Foundation of China and Henan Province ( U1304202 ), Henan Province Science and Technology Project ( 222300420523, 212102210646) and The Scientific Research Innovation Team of Xuchang University ( 2022CXTD001 ) for financial support.

Funders	Funder number
Fostering Talents of National Natural Science Foundation of China and Henan Province	U1304202, 212102210646, 222300420523
Jesus College Oxford
Scientific Research Innovation Team of Xuchang University	2022CXTD001
Henan Normal University	2020ZD01
Engineering and Physical Sciences Research Council	EP/V00462X/1
University of Oxford
University of Bath
National Natural Science Foundation of China	21001040, 21907023
Science and Technology Program of Hunan Province	232102320092

Keywords

Biological imaging
Carboxylesterases
Fluorescence
Probe
Sensors

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

10.1016/j.snb.2023.135121Licence: CC BY

Cite this

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title = "“Turn-on” and ratiometric detection of carboxylesterases using acetyl-based fluorescent strategies for bacterial applications",

abstract = "Carboxylesterases (CEs) are involved in the biotransformation of numerous drugs, environmental toxicants, and carcinogens containing ester groups. Abnormal CEs levels are associated with various diseases, and as such they are widely studied in cells. CEs are also characteristic biomarkers for assisting in the detection of certain strains of bacteria and are involved in important biological processes. Herein, we used two probes including an indene-chalcone-based fluorescent probe which displays green “turn-on” fluorescence response, and a merocyanine-based ratiometric probe to detect CEs, as well as detect and image S. aureus and their chronic biofilms using acetate ester as recognition group.",

keywords = "Biological imaging, Carboxylesterases, Fluorescence, Probe, Sensors",

author = "Yan, {Kai Cheng} and Guo, {Fang Fang} and Ling Zhang and Naing Thet and Sedgwick, {Adam C.} and Yuan Wang and Xu, {Zhi Hong} and James, {Tony D.} and Jenkins, {A. Toby A.}",

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AU - Yan, Kai Cheng

AU - Guo, Fang Fang

AU - Zhang, Ling

AU - Thet, Naing

AU - Sedgwick, Adam C.

AU - Wang, Yuan

AU - Xu, Zhi Hong

AU - James, Tony D.

AU - Jenkins, A. Toby A.

PY - 2024/4/1

Y1 - 2024/4/1

N2 - Carboxylesterases (CEs) are involved in the biotransformation of numerous drugs, environmental toxicants, and carcinogens containing ester groups. Abnormal CEs levels are associated with various diseases, and as such they are widely studied in cells. CEs are also characteristic biomarkers for assisting in the detection of certain strains of bacteria and are involved in important biological processes. Herein, we used two probes including an indene-chalcone-based fluorescent probe which displays green “turn-on” fluorescence response, and a merocyanine-based ratiometric probe to detect CEs, as well as detect and image S. aureus and their chronic biofilms using acetate ester as recognition group.

AB - Carboxylesterases (CEs) are involved in the biotransformation of numerous drugs, environmental toxicants, and carcinogens containing ester groups. Abnormal CEs levels are associated with various diseases, and as such they are widely studied in cells. CEs are also characteristic biomarkers for assisting in the detection of certain strains of bacteria and are involved in important biological processes. Herein, we used two probes including an indene-chalcone-based fluorescent probe which displays green “turn-on” fluorescence response, and a merocyanine-based ratiometric probe to detect CEs, as well as detect and image S. aureus and their chronic biofilms using acetate ester as recognition group.

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KW - Carboxylesterases

KW - Fluorescence

KW - Probe

KW - Sensors

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M3 - Article

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“Turn-on” and ratiometric detection of carboxylesterases using acetyl-based fluorescent strategies for bacterial applications

Abstract

Funding

Keywords

ASJC Scopus subject areas

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Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

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“Turn-on” and ratiometric detection of carboxylesterases using acetyl-based fluorescent strategies for bacterial applications

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Cite this