Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre

Ben Sherlock, Fei Yu, Jim Stone, Sean Warren, Carl Paterson, Mark A. A. Neil, Paul M. W. French, Jonathan Knight, Chris Dunsby

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Negative curvature fibre (NCF) guides light in its core by inhibiting the coupling of core and cladding modes. In this work, an NCF was designed and fabricated to transmit ultrashort optical pulses for multiphoton microscopy with low group velocity dispersion (GVD) at 800 nm. Its attenuation was measured to be <0.3 dB.m-1 over the range 600-850 nm and the GVD was -180±70 fs2.m-1 at 800 nm. Using an average fibre output power of ~20 mW and pulse repetition rate of 80 MHz, the NCF enabled pulses with a duration of <200 fs to be transmitted through a length of 1.5 m of fibre over a tuning range of 180 nm without the need for dispersion compensation. In a 4 m fibre, temporal and spectral pulse widths were maintained to within 10% of low power values up to the maximum fibre output power achievable with the laser system used of 278 mW at 700 nm, 808 mW at 800 nm and 420 mW at 860 nm. When coupled to a multiphoton microscope, it enabled imaging of ex vivo tissue using excitation wavelengths from 740 nm to 860 nm without any need for adjustments to the set-up.
Original languageEnglish
Pages (from-to)715-720
JournalJournal of Biophotonics
Volume9
Issue number7
Early online date15 Mar 2016
DOIs
Publication statusPublished - Jul 2016

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Optical fiber coupling
Microscopy
Microscopic examination
Lasers
Heart Rate
curvature
microscopy
Light
fibers
Fibers
Group velocity dispersion
group velocity
Pulse repetition rate
Dispersion compensation
output
pulse repetition rate
pulses
low speed
Laser pulses
pulse duration

Cite this

Sherlock, B., Yu, F., Stone, J., Warren, S., Paterson, C., Neil, M. A. A., ... Dunsby, C. (2016). Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre. Journal of Biophotonics, 9(7), 715-720. https://doi.org/10.1002/jbio.201500290

Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre. / Sherlock, Ben; Yu, Fei; Stone, Jim; Warren, Sean; Paterson, Carl; Neil, Mark A. A.; French, Paul M. W.; Knight, Jonathan; Dunsby, Chris.

In: Journal of Biophotonics, Vol. 9, No. 7, 07.2016, p. 715-720.

Research output: Contribution to journalArticle

Sherlock, B, Yu, F, Stone, J, Warren, S, Paterson, C, Neil, MAA, French, PMW, Knight, J & Dunsby, C 2016, 'Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre', Journal of Biophotonics, vol. 9, no. 7, pp. 715-720. https://doi.org/10.1002/jbio.201500290
Sherlock, Ben ; Yu, Fei ; Stone, Jim ; Warren, Sean ; Paterson, Carl ; Neil, Mark A. A. ; French, Paul M. W. ; Knight, Jonathan ; Dunsby, Chris. / Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre. In: Journal of Biophotonics. 2016 ; Vol. 9, No. 7. pp. 715-720.
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