TY - JOUR
T1 - Tripodal BODIPY-Tagged and Functional Molecular Probes
T2 - Synthesis, Computational Investigations and Explorations by Multiphoton Fluorescence Lifetime Imaging Microscopy
AU - Lledos, Marina
AU - Gonzalez Calatayud, David
AU - Cortezon-Tamarit, Fernando
AU - Ge, Haobo
AU - Pourzand, Charareh
AU - Botchway, Stanley W.
AU - Sodupe, Mariona
AU - Lledos, Agusti
AU - Eggleston, Ian
AU - Pascu, Sofia I.
PY - 2024/9/2
Y1 - 2024/9/2
N2 - A range of novel BODIPY derivatives with a tripodal aromatic core was synthesized and characterized spectroscopically. These new fluorophores showed promising features as probes for in vitro assays in live cells and offer strategic routes for further functionalization towards hybrid nanomaterials. Incorporation of biotin tags facilitated proof-of-concept access to targeted bioconjugates as molecular probes. Computational explorations using DFT and TD-DFT calculations identified the most stable tripodal linker conformations and predicted their absorption and emission behavior. The uptake and speciation of these molecules in living prostate cancer cells was imaged by single- and two-photon excitation techniques coupled with two-photon fluorescence lifetime imaging (2P FLIM).
AB - A range of novel BODIPY derivatives with a tripodal aromatic core was synthesized and characterized spectroscopically. These new fluorophores showed promising features as probes for in vitro assays in live cells and offer strategic routes for further functionalization towards hybrid nanomaterials. Incorporation of biotin tags facilitated proof-of-concept access to targeted bioconjugates as molecular probes. Computational explorations using DFT and TD-DFT calculations identified the most stable tripodal linker conformations and predicted their absorption and emission behavior. The uptake and speciation of these molecules in living prostate cancer cells was imaged by single- and two-photon excitation techniques coupled with two-photon fluorescence lifetime imaging (2P FLIM).
KW - Confocal microscopy and Airyscan super resolution
KW - Multiphoton FLIM
KW - TD-DFT calculations
KW - conjugatable tripodal D-biotin conjugates
KW - multiplexing with BODIPY tags
UR - http://www.scopus.com/inward/record.url?scp=85200990719&partnerID=8YFLogxK
U2 - 10.1002/chem.202400858
DO - 10.1002/chem.202400858
M3 - Article
SN - 0947-6539
VL - 30
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
IS - 49
M1 - e202400858
ER -
