Background: Haemophilus influenzae requires a substrate-binding protein (SBP)-dependent TRAP transporter to acquire sialic acid. Results: A conserved arginine residue in the SBP is essential for the high affinity and carboxylate specificity of the TRAP transporter. Conclusion: The arginine/carboxylate interaction in TRAP SBPs restricts substrate range to carboxylate-containing substrates. Significance: The study reveals the mechanism by which a key bimolecular interaction underpins bacterial virulence.
|Number of pages||11|
|Journal||Journal of Biological Chemistry|
|Early online date||5 Sep 2015|
|Publication status||Published - Nov 2015|
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- Department of Life Sciences - Senior Lecturer
- Centre for Therapeutic Innovation
Person: Research & Teaching