Tri-mode optical biopsy probe with fluorescence endomicroscopy, Raman spectroscopy, and time-resolved fluorescence spectroscopy

Harry Alexander Charles Wood, Katjana Ehrlich, Stephanos Yerolatsitis, András Kufcsák, Tom Michael Quinn, Susan Fernandes, Dominic Norberg, Nia Caitlin Jenkins, Vikki Young, Irene Young, Katie Hamilton, Sohan Seth, Ahsan Akram, Robert Rodrick Thomson, Keith Finlayson, Kevin Dhaliwal, James Morgan Stone

Research output: Contribution to journalArticlepeer-review

2 Citations (SciVal)


We present an endoscopic probe that combines three distinct optical fibre technologies including: A high-resolution imaging fibre for optical endomicroscopy, a multimode fibre for time-resolved fluorescence spectroscopy, and a hollow-core fibre with multimode signal collection cores for Raman spectroscopy. The three fibers are all enclosed within a 1.2 mm diameter clinical grade catheter with a 1.4 mm end cap. To demonstrate the probe's flexibility we provide data acquired with it in loops of radii down to 2 cm. We then use the probe in an anatomically accurate model of adult human airways, showing that it can be navigated to any part of the distal lung using a commercial bronchoscope. Finally, we present data acquired from fresh ex vivo human lung tissue. Our experiments show that this minimally invasive probe can deliver real-time optical biopsies from within the distal lung - simultaneously acquiring co-located high-resolution endomicroscopy and biochemical spectra.

Original languageEnglish
Article numbere202200141
JournalJournal of Biophotonics
Issue number2
Early online date5 Sept 2022
Publication statusPublished - 28 Feb 2023

Bibliographical note

Funding Information:
This work was supported by the UKRI grants EP/S001123/1 and MR/R017794/1, and an award from BTG/Boston Scientific.


  • fluorescence endomicroscopy
  • micro endoscopy
  • Raman endoscopy
  • time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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