Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship; Peter J. Watson; Nahid Rahman-Huq; Louise Fairall; Mareike G. Posner; Abhishek Upadhyay; Yamini Reddivari; Jonathan M G Chamberlain; Simon E. Kolstoe; Stefan Bagby; John W R Schwabe; Raymond J. Owens

doi:10.1007/978-1-4939-2205-5_11

Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship
, Peter J. Watson
, Nahid Rahman-Huq
, Louise Fairall
, Mareike G. Posner
, Abhishek Upadhyay
, Yamini Reddivari
, Jonathan M G Chamberlain
, Simon E. Kolstoe
, Stefan Bagby
, John W R Schwabe
, Raymond J. Owens

Department of Life Sciences

Rutherford Appleton Laboratory
University of Oxford
University of Leicester
University of Portsmouth

Research output: Contribution to journal › Article › peer-review

13 Citations (SciVal)

2551 Downloads (Pure)

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Original language	English
Pages (from-to)	209-222
Number of pages	14
Journal	Methods in Molecular Biology
Volume	1258
DOIs	https://doi.org/10.1007/978-1-4939-2205-5_11
Publication status	Published - 16 Nov 2014

Keywords

FLAG purification
HEK
Intracellular protein
Mammalian
Protein complexes
Secreted protein
Transient transfection

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10.1007/978-1-4939-2205-5_11

Mammalian Proteins_FinalAccepted author manuscript, 701 KB

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Nettleship, J. E., Watson, P. J., Rahman-Huq, N., Fairall, L., Posner, M. G., Upadhyay, A., Reddivari, Y., Chamberlain, J. M. G., Kolstoe, S. E., Bagby, S., Schwabe, J. W. R., & Owens, R. J. (2014). Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins. Methods in Molecular Biology, 1258, 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11

Nettleship, JE, Watson, PJ, Rahman-Huq, N, Fairall, L, Posner, MG, Upadhyay, A, Reddivari, Y, Chamberlain, JMG, Kolstoe, SE, Bagby, S, Schwabe, JWR & Owens, RJ 2014, 'Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins', Methods in Molecular Biology, vol. 1258, pp. 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11

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title = "Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins",

abstract = "Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.",

keywords = "FLAG purification, HEK, Intracellular protein, Mammalian, Protein complexes, Secreted protein, Transient transfection",

author = "Nettleship, \{Joanne E.\} and Watson, \{Peter J.\} and Nahid Rahman-Huq and Louise Fairall and Posner, \{Mareike G.\} and Abhishek Upadhyay and Yamini Reddivari and Chamberlain, \{Jonathan M G\} and Kolstoe, \{Simon E.\} and Stefan Bagby and Schwabe, \{John W R\} and Owens, \{Raymond J.\}",

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month = nov,

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doi = "10.1007/978-1-4939-2205-5\_11",

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T2 - An alternative to E. coli for the production of secreted and intracellular mammalian proteins

AU - Nettleship, Joanne E.

AU - Watson, Peter J.

AU - Rahman-Huq, Nahid

AU - Fairall, Louise

AU - Posner, Mareike G.

AU - Upadhyay, Abhishek

AU - Reddivari, Yamini

AU - Chamberlain, Jonathan M G

AU - Kolstoe, Simon E.

AU - Bagby, Stefan

AU - Schwabe, John W R

AU - Owens, Raymond J.

PY - 2014/11/16

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N2 - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

AB - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

KW - FLAG purification

KW - HEK

KW - Intracellular protein

KW - Mammalian

KW - Protein complexes

KW - Secreted protein

KW - Transient transfection

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UR - http://dx.doi.org/10.1007/978-1-4939-2205-5_11

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SP - 209

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Abstract

Keywords

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Structural, Biochemical and Computational Studies of KIBRA Protein-Protein and Membrane Interactions

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Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Abstract

Keywords

Access to Document

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Projects

Structural, Biochemical and Computational Studies of KIBRA Protein-Protein and Membrane Interactions

Cite this