Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M G Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W R Schwabe, Raymond J. Owens

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Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Original languageEnglish
Pages (from-to)209-222
Number of pages14
JournalMethods in Molecular Biology
Volume1258
DOIs
Publication statusPublished - 16 Nov 2014

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Keywords

  • FLAG purification
  • HEK
  • Intracellular protein
  • Mammalian
  • Protein complexes
  • Secreted protein
  • Transient transfection

Cite this

Nettleship, J. E., Watson, P. J., Rahman-Huq, N., Fairall, L., Posner, M. G., Upadhyay, A., ... Owens, R. J. (2014). Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins. Methods in Molecular Biology, 1258, 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11