Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M G Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W R Schwabe, Raymond J. Owens

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

LanguageEnglish
Pages209-222
Number of pages14
JournalMethods in Molecular Biology
Volume1258
DOIs
StatusPublished - 16 Nov 2014

Fingerprint

HEK293 Cells
Escherichia coli
Recombinant Proteins
Transfection
Proteins
Kidney

Keywords

  • FLAG purification
  • HEK
  • Intracellular protein
  • Mammalian
  • Protein complexes
  • Secreted protein
  • Transient transfection

Cite this

Nettleship, J. E., Watson, P. J., Rahman-Huq, N., Fairall, L., Posner, M. G., Upadhyay, A., ... Owens, R. J. (2014). Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins. Methods in Molecular Biology, 1258, 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11

Transient expression in HEK 293 cells : An alternative to E. coli for the production of secreted and intracellular mammalian proteins. / Nettleship, Joanne E.; Watson, Peter J.; Rahman-Huq, Nahid; Fairall, Louise; Posner, Mareike G.; Upadhyay, Abhishek; Reddivari, Yamini; Chamberlain, Jonathan M G; Kolstoe, Simon E.; Bagby, Stefan; Schwabe, John W R; Owens, Raymond J.

In: Methods in Molecular Biology, Vol. 1258, 16.11.2014, p. 209-222.

Research output: Contribution to journalArticle

Nettleship, JE, Watson, PJ, Rahman-Huq, N, Fairall, L, Posner, MG, Upadhyay, A, Reddivari, Y, Chamberlain, JMG, Kolstoe, SE, Bagby, S, Schwabe, JWR & Owens, RJ 2014, 'Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins', Methods in Molecular Biology, vol. 1258, pp. 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11
Nettleship, Joanne E. ; Watson, Peter J. ; Rahman-Huq, Nahid ; Fairall, Louise ; Posner, Mareike G. ; Upadhyay, Abhishek ; Reddivari, Yamini ; Chamberlain, Jonathan M G ; Kolstoe, Simon E. ; Bagby, Stefan ; Schwabe, John W R ; Owens, Raymond J. / Transient expression in HEK 293 cells : An alternative to E. coli for the production of secreted and intracellular mammalian proteins. In: Methods in Molecular Biology. 2014 ; Vol. 1258. pp. 209-222.
@article{6e2bc85f01e64703adee76644ca23219,
title = "Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins",
abstract = "Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.",
keywords = "FLAG purification, HEK, Intracellular protein, Mammalian, Protein complexes, Secreted protein, Transient transfection",
author = "Nettleship, {Joanne E.} and Watson, {Peter J.} and Nahid Rahman-Huq and Louise Fairall and Posner, {Mareike G.} and Abhishek Upadhyay and Yamini Reddivari and Chamberlain, {Jonathan M G} and Kolstoe, {Simon E.} and Stefan Bagby and Schwabe, {John W R} and Owens, {Raymond J.}",
year = "2014",
month = "11",
day = "16",
doi = "10.1007/978-1-4939-2205-5_11",
language = "English",
volume = "1258",
pages = "209--222",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Transient expression in HEK 293 cells

T2 - Methods in Molecular Biology

AU - Nettleship, Joanne E.

AU - Watson, Peter J.

AU - Rahman-Huq, Nahid

AU - Fairall, Louise

AU - Posner, Mareike G.

AU - Upadhyay, Abhishek

AU - Reddivari, Yamini

AU - Chamberlain, Jonathan M G

AU - Kolstoe, Simon E.

AU - Bagby, Stefan

AU - Schwabe, John W R

AU - Owens, Raymond J.

PY - 2014/11/16

Y1 - 2014/11/16

N2 - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

AB - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable labscale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

KW - FLAG purification

KW - HEK

KW - Intracellular protein

KW - Mammalian

KW - Protein complexes

KW - Secreted protein

KW - Transient transfection

UR - http://www.scopus.com/inward/record.url?scp=84916237753&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1007/978-1-4939-2205-5_11

U2 - 10.1007/978-1-4939-2205-5_11

DO - 10.1007/978-1-4939-2205-5_11

M3 - Article

VL - 1258

SP - 209

EP - 222

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -