Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres

M D VerMilyea, M Maneck, N Yoshida, I Blochberger, E Suzuki, Toru Suzuki, R Spang, C A Klein, Anthony C F Perry

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38 Citations (Scopus)

Abstract

Transcriptome regionalization is an essential polarity determinant among metazoans, directing embryonic axis formation during normal development. Although conservation of this principle in mammals is assumed, recent evidence is conflicting and it is not known whether transcriptome asymmetries exist within unfertilized mammalian eggs or between the respective cleavage products of early embryonic divisions. We here address this by comparing transcriptome profiles of paired single cells and sub-cellular structures obtained microsurgically from mouse oocytes and totipotent embryos. Paired microsurgical spindle and remnant samples from unfertilized metaphase II oocytes possessed distinguishable profiles. Fertilization produces a totipotent 1-cell embryo (zygote) and associated spindle-enriched second polar body whose paired profiles also differed, reflecting spindle transcript enrichment. However, there was no programmed transcriptome asymmetry between sister cells within 2-or 3-cell embryos. Accordingly, there is transcriptome asymmetry within mouse oocytes, but not between the sister blastomeres of early embryos. This work places constraints on pre-patterning in mammals and provides documentation correlating potency changes and transcriptome partitioning at the single-cell level.
Original languageEnglish
Pages (from-to)1841-1851
Number of pages11
JournalEMBO Journal
Volume30
Issue number9
Early online date4 Apr 2011
DOIs
Publication statusPublished - 4 May 2011

Keywords

  • pre-patterning
  • polarity
  • transcriptome
  • pre-implantation embryo
  • mouse metaphase II

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    VerMilyea, M. D., Maneck, M., Yoshida, N., Blochberger, I., Suzuki, E., Suzuki, T., Spang, R., Klein, C. A., & Perry, A. C. F. (2011). Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres. EMBO Journal, 30(9), 1841-1851. https://doi.org/10.1038/emboj.2011.92