Transactivation of mouse insulin-like growth factor II (IGF-II) gene promoters by the AP-1 complex

A A Caricasole, A Ward

Research output: Contribution to journalArticle

Abstract

The mouse insulin-like growth factor II gene (Igf2) is transcribed from three promoters (P1, P2 and P3), and is expressed in a tissue-specific and developmentally regulated fashion; however, little information is available on the transcription factors controlling Igf2 expression. The AP-1 complex is a transcription factor involved in the regulation of a variety of genes, including those encoding certain growth factors. We show that Igf2 P3 is transactivated by AP-1 in a transient expression assay, and that this effect is mediated through two non-consensus AP-1 binding sites characterised by DNA-protein interaction studies. Mutational analysis indicates these sites are required for AP-1 responsiveness and full promoter activity.

Original languageEnglish
Pages (from-to)1873-9
Number of pages7
JournalNucleic Acids Research
Volume21
Issue number8
Publication statusPublished - 25 Apr 1993

Keywords

  • Animals
  • Base Sequence
  • Binding Sites
  • Blotting, Northern
  • Cell Differentiation
  • Cell Line
  • DNA
  • Insulin-Like Growth Factor II
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins c-jun
  • Rats
  • Stem Cells
  • Transcriptional Activation
  • Journal Article
  • Research Support, Non-U.S. Gov't

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