Trafficking and signalling of gonadotrophin-releasing hormone receptors: an automated imaging approach

A R Finch, K R Sedgley, S P Armstrong, C J Caunt, C A McArdle

Research output: Contribution to journalLiterature review

  • 9 Citations

Abstract

Gonadotrophin-releasing hormone (GnRH) is a neuropeptide that mediates central control of reproduction by stimulating gonadotrophin secretion from the pituitary. It acts via 7 transmembrane region (7TM) receptors that lack C-terminal tails, regions that for many 7TM receptors, are necessary for agonist-induced phosphorylation and arrestin binding as well as arrestin-dependent desensitization, internalization and signalling. Recent work has revealed that human GnRH receptors (GnRHR) are poorly expressed at the cell surface. This apparently reflects inefficient exit from the endoplasmic reticulum, which is thought to be increased by pharmacological chaperones (non-peptide GnRHR antagonists that increase cell surface GnRHR expression) or reduced by point mutations that further impair GnRHR trafficking and thereby cause infertility. Here, we review recent work in this field, with emphasis on the use of semi-automated imaging to interrogate compartmentalization and trafficking of these unique 7TM receptors
LanguageEnglish
Pages751-760
Number of pages10
JournalBritish Journal of Pharmacology
Volume159
Issue number4
DOIs
StatusPublished - Feb 2010

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LHRH Receptors
Arrestin
Pituitary Gonadotropins
Cell Surface Receptors
Neuropeptides
Point Mutation
Gonadotropin-Releasing Hormone
Endoplasmic Reticulum
Infertility
Reproduction
Phosphorylation
Pharmacology

Cite this

Finch, A. R., Sedgley, K. R., Armstrong, S. P., Caunt, C. J., & McArdle, C. A. (2010). Trafficking and signalling of gonadotrophin-releasing hormone receptors: an automated imaging approach. DOI: 10.1111/j.1476-5381.2009.00413.x

Trafficking and signalling of gonadotrophin-releasing hormone receptors : an automated imaging approach. / Finch, A R; Sedgley, K R; Armstrong, S P; Caunt, C J; McArdle, C A.

In: British Journal of Pharmacology, Vol. 159, No. 4, 02.2010, p. 751-760.

Research output: Contribution to journalLiterature review

Finch, A R ; Sedgley, K R ; Armstrong, S P ; Caunt, C J ; McArdle, C A. / Trafficking and signalling of gonadotrophin-releasing hormone receptors : an automated imaging approach. In: British Journal of Pharmacology. 2010 ; Vol. 159, No. 4. pp. 751-760
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