Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development

Marta Duch, Núria Torras, Maki Asami, Toru Suzuki, María Arjona, Rodrigo Gómez-Martínez, Matthew VerMilyea, Robert Castilla, José Antonio Plaza, Anthony Perry

Research output: Contribution to journalArticle

Abstract

Cells comprise mechanically active matter that governs their functionality, but intracellular mechanics are difficult to study directly and are poorly understood. However, injected nanodevices open up opportunities to analyse intracellular mechanobiology. Here, we identify a programme of forces and changes to the cytoplasmic mechanical properties required for mouse embryo development from fertilization to the first cell division. Injected, fully internalized nanodevices responded to sperm decondensation and recondensation, and subsequent device behaviour suggested a model for pronuclear convergence based on a gradient of effective cytoplasmic stiffness. The nanodevices reported reduced cytoplasmic mechanical activity during chromosome alignment and indicated that cytoplasmic stiffening occurred during embryo elongation, followed by rapid cytoplasmic softening during cytokinesis (cell division). Forces greater than those inside muscle cells were detected within embryos. These results suggest that intracellular forces are part of a concerted programme that is necessary for development at the origin of a new embryonic life.
Original languageEnglish
Number of pages14
JournalNature Materials
Early online date25 May 2020
DOIs
Publication statusE-pub ahead of print - 25 May 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Duch, M., Torras, N., Asami, M., Suzuki, T., Arjona, M., Gómez-Martínez, R., VerMilyea, M., Castilla, R., Plaza, J. A., & Perry, A. (2020). Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development. Nature Materials. https://doi.org/10.1038/s41563-020-0685-9