Towards timely Alzheimer diagnosis: A self-powered amperometric biosensor for the neurotransmitter acetylcholine

Felismina T.C. Moreira, M. Goreti F. Sale, Mirella Di Lorenzo

Research output: Contribution to journalArticle

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Abstract

Serious brain disorders, such as the Alzheimer's Disease (AD), are associated with a marked drop in the levels of important neurotransmitters, such as acetylcholine (ACh). Real time monitoring of such biomarkers can therefore play a critical role in enhancing AD therapies by allowing timely diagnosis, verifications of treatment effectiveness, and developments of new medicines. In this study, we present the first acetylcholine/oxygen hybrid enzymatic fuel cell for the self-powered on site detection of ACh in plasma, which is based on the combination of an enzymatic anode with a Pt cathode. Firstly, an effective acetylcholinesterase immobilized electrode was developed and its electrochemical performance evaluated. Highly porous gold was used as the electrode material, and the enzyme was immobilized via a one step rapid and simple procedure that does not require the use of harsh chemicals or any electrode/enzyme pre-treatments. The resulting enzymatic electrode was subsequently used as the anode of a miniature flow-through membrane-less fuel cell and showed excellent response to varying concentrations of ACh. The peak power generated by the fuel cell was 4 nW at a voltage of 260 mV and with a current density of 9 μA cm−2. The limit of detection of the fuel cell sensor was 10 μM, with an average response time as short as 3 min. These exciting results open new horizons for point-of-care Alzheimer diagnosis and provide an attractive potential alternative to established methods that require laborious and time-consuming sample treatments and expensive instruments.
LanguageEnglish
Pages607-614
JournalBiosensors and Bioelectronics
Volume87
Early online date30 Aug 2016
DOIs
StatusPublished - 15 Jan 2017

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Biosensing Techniques
Biosensors
Acetylcholine
Neurotransmitter Agents
Fuel cells
Electrodes
Enzymatic fuel cells
Anodes
Enzyme electrodes
Biomarkers
Alzheimer Disease
Medicine
Point-of-Care Systems
Brain
Cathodes
Current density
Acetylcholinesterase
Enzymes
Gold
Immobilized Enzymes

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Towards timely Alzheimer diagnosis: A self-powered amperometric biosensor for the neurotransmitter acetylcholine. / Moreira, Felismina T.C.; Sale, M. Goreti F.; Di Lorenzo, Mirella.

In: Biosensors and Bioelectronics, Vol. 87, 15.01.2017, p. 607-614.

Research output: Contribution to journalArticle

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