We demonstrate a fiber-based two-color source of picosecond pulses for coherent Raman scattering (CRS) microscopy. An Yb-doped fiber laser combined with a divided-pulse amplifier produce up to 3 W of power tunable from 1030 nm to 1040 nm. A normal dispersion photonic crystal fiber is used to blue-shift the pulses through seeded four-wave mixing. Pulses with up to 150 mW of average power are produced, tunable between 770 nm and 800 nm. Imaging of animal tissue and cells is demonstrated.
Lefrancois, S., Fu, D., Holtom, G., Kong, L., Wadsworth, W., Schneider, P., Herda, R., Zach, A., Xie, X. S., & Wise, F. W. (2012). Towards a tunable fiber source for coherent Raman imaging. Proceedings of the SPIE, 8226, . https://doi.org/10.1117/12.920187