Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen

Dipti Rani, Vivek Pachauri, N. Madaboosi, Pawan Jolly, Xuan-Thang Vu, Pedro Estrela, Virginia Chu, Joao Pedro Conde, Sven Ingebrandt

Research output: Contribution to journalArticle

2 Citations (Scopus)
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Abstract

Highly sensitive electrical detection of biomarkers for the early stage screening of cancer is desired for future, ultrafast diagnostic platforms. In the case of prostate cancer (PCa), the prostate-specific antigen (PSA) is of prime interest and its detection in combination with other PCa-relevant biomarkers in a multiplex approach is advised. Toward this goal, we demonstrate the label-free, potentiometric detection of PSA with silicon nanowire ion-sensitive field-effect transistor (Si NW-ISFET) arrays. To realize the field-effect detection, we utilized the DNA aptamer-receptors specific for PSA, which were covalently and site-specifically immobilized on Si NW-ISFETs. The platform was used for quantitative detection of PSA and the change in threshold voltage of the Si NW-ISEFTs was correlated with the concentration of PSA. Concentration-dependent measurements were done in a wide range of 1 pg/mL to 1 μg/mL, which covers the clinical range of interest. To confirm the PSA–DNA aptamer binding on the Si NW surfaces, a sandwich-immunoassay based on chemiluminescence was implemented. The electrical approach using the Si NW-ISFET platform shows a lower limit of detection and a wide dynamic range of the assay. In future, our platform should be utilized to detect multiple biomarkers in one assay to obtain more reliable information about cancer-related diseases.
Original languageEnglish
Pages (from-to)8471-8482
JournalACS OMEGA
Volume3
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

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Silicon
Prostate-Specific Antigen
Antigens
Nanowires
Ion sensitive field effect transistors
Nucleotide Aptamers
Biomarkers
Assays
DNA
Chemiluminescence
Threshold voltage
Labels
Screening

Cite this

Rani, D., Pachauri, V., Madaboosi, N., Jolly, P., Vu, X-T., Estrela, P., ... Ingebrandt, S. (2018). Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen. ACS OMEGA, 3(8), 8471-8482. https://doi.org/10.1021/acsomega.8b00990

Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen. / Rani, Dipti; Pachauri, Vivek; Madaboosi, N.; Jolly, Pawan; Vu, Xuan-Thang; Estrela, Pedro; Chu, Virginia; Conde, Joao Pedro; Ingebrandt, Sven.

In: ACS OMEGA, Vol. 3, No. 8, 01.08.2018, p. 8471-8482.

Research output: Contribution to journalArticle

Rani, D, Pachauri, V, Madaboosi, N, Jolly, P, Vu, X-T, Estrela, P, Chu, V, Conde, JP & Ingebrandt, S 2018, 'Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen', ACS OMEGA, vol. 3, no. 8, pp. 8471-8482. https://doi.org/10.1021/acsomega.8b00990
Rani, Dipti ; Pachauri, Vivek ; Madaboosi, N. ; Jolly, Pawan ; Vu, Xuan-Thang ; Estrela, Pedro ; Chu, Virginia ; Conde, Joao Pedro ; Ingebrandt, Sven. / Top-Down Fabricated Silicon Nanowire Arrays for Field-Effect Detection of Prostate-Specific Antigen. In: ACS OMEGA. 2018 ; Vol. 3, No. 8. pp. 8471-8482.
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