Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP

T A Masters; N A Robinson; R J Marsh; T S Blacker; D A Armoogum; B Larijani; A J Bain

doi:10.1063/1.5011643

Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP

T A Masters, N A Robinson, R J Marsh, T S Blacker, D A Armoogum, B Larijani, A J Bain

Research output: Contribution to journal › Article › peer-review

3 Citations (SciVal)

Abstract

Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-Förster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.

Original language	English
Article number	134312
Journal	Journal of Chemical Physics
Volume	148
Issue number	13
Early online date	6 Apr 2018
DOIs	https://doi.org/10.1063/1.5011643
Publication status	E-pub ahead of print - 6 Apr 2018

Keywords

Fluorescence Polarization
Fluorescence Resonance Energy Transfer
Green Fluorescent Proteins/chemistry
Light
Models, Chemical
Photons
Thermodynamics

Access to Document

10.1063/1.5011643Licence: CC BY

Cite this

@article{a4e9aef45ae44f578d3c89638cd48a9d,

title = "Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP",

abstract = "Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-F{\"o}rster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.",

keywords = "Fluorescence Polarization, Fluorescence Resonance Energy Transfer, Green Fluorescent Proteins/chemistry, Light, Models, Chemical, Photons, Thermodynamics",

author = "Masters, {T A} and Robinson, {N A} and Marsh, {R J} and Blacker, {T S} and Armoogum, {D A} and B Larijani and Bain, {A J}",

year = "2018",

month = apr,

day = "6",

doi = "10.1063/1.5011643",

language = "English",

volume = "148",

journal = "Journal of Chemical Physics",

issn = "0021-9606",

publisher = "American Institute of Physics",

number = "13",

}

TY - JOUR

T1 - Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP

AU - Masters, T A

AU - Robinson, N A

AU - Marsh, R J

AU - Blacker, T S

AU - Armoogum, D A

AU - Larijani, B

AU - Bain, A J

PY - 2018/4/6

Y1 - 2018/4/6

N2 - Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-Förster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.

AB - Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-Förster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.

KW - Fluorescence Polarization

KW - Fluorescence Resonance Energy Transfer

KW - Green Fluorescent Proteins/chemistry

KW - Light

KW - Models, Chemical

KW - Photons

KW - Thermodynamics

U2 - 10.1063/1.5011643

DO - 10.1063/1.5011643

M3 - Article

C2 - 29626854

SN - 0021-9606

VL - 148

JO - Journal of Chemical Physics

JF - Journal of Chemical Physics

IS - 13

M1 - 134312

ER -

Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP

Abstract

Keywords

Access to Document

Fingerprint

Cite this