Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP

T A Masters, N A Robinson, R J Marsh, T S Blacker, D A Armoogum, B Larijani, A J Bain

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-Förster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.

Original languageEnglish
Article number134312
JournalJournal of Chemical Physics
Volume148
Issue number13
Early online date6 Apr 2018
DOIs
Publication statusE-pub ahead of print - 6 Apr 2018

Keywords

  • Fluorescence Polarization
  • Fluorescence Resonance Energy Transfer
  • Green Fluorescent Proteins/chemistry
  • Light
  • Models, Chemical
  • Photons
  • Thermodynamics

Cite this

Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP. / Masters, T A; Robinson, N A; Marsh, R J; Blacker, T S; Armoogum, D A; Larijani, B; Bain, A J.

In: Journal of Chemical Physics, Vol. 148, No. 13, 134312, 06.04.2018.

Research output: Contribution to journalArticle

Masters, T A ; Robinson, N A ; Marsh, R J ; Blacker, T S ; Armoogum, D A ; Larijani, B ; Bain, A J. / Time-resolved stimulated emission depletion and energy transfer dynamics in two-photon excited EGFP. In: Journal of Chemical Physics. 2018 ; Vol. 148, No. 13.
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AU - Marsh, R J

AU - Blacker, T S

AU - Armoogum, D A

AU - Larijani, B

AU - Bain, A J

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AB - Time and polarization-resolved stimulated emission depletion (STED) measurements are used to investigate excited state evolution following the two-photon excitation of enhanced green fluorescent protein (EGFP). We employ a new approach for the accurate STED measurement of the hitherto unmeasured degree of hexadecapolar transition dipole moment alignment α40 present at a given excitation-depletion (pump-dump) pulse separation. Time-resolved polarized fluorescence measurements as a function of pump-dump delay reveal the time evolution of α40 to be considerably more rapid than predicted for isotropic rotational diffusion in EGFP. Additional depolarization by homo-Förster resonance energy transfer is investigated for both α20 (quadrupolar) and α40 transition dipole alignments. These results point to the utility of higher order dipole correlation measurements in the investigation of resonance energy transfer processes.

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KW - Green Fluorescent Proteins/chemistry

KW - Light

KW - Models, Chemical

KW - Photons

KW - Thermodynamics

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