Three-dimensional ultrastructure of the ring stage of Plasmodium falciparum: Evidence for export pathways

Lawrence Howard Bannister, John Mervyn Hopkins, Gabriele Margos, Anton Richard Dluzewski, Graham Howard Mitchell

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The three-dimensional structure of the Plasmodium falciparum ring stage has been explored by reconstruction from serial sections and stereoscopic examination of tilted sections. The ring-like light microscopic appearance is related to the shape and contents of the biconcave discoidal parasite at this stage, its thick perimeter containing most of the ribosomes and its thin center containing smooth membrane organelles. The shapes of rings vary between flat and curved cuplike forms. The rough endoplasmic reticulum is a branched network continuous with the nuclear envelope. Evidence for a simple Golgi complex is seen in the presence on the outer nuclear envelope of a locus of coated vesicle budding associated with a single membranous cisterna or cluster of smooth vesicles. In middle and late stage rings this complex migrates along an extension of the nuclear envelope continuous with the rough endoplasmic reticulum. Evidence is also presented for a mechanism of exporting membrane from the parasite into the parasitophorous vacuole membrane and beyond into the red blood cell, by means of double-membraned vesicle-based exocytosis.
Original languageEnglish
Pages (from-to)551-562
Number of pages12
JournalMicroscopy and Microanalysis
Volume10
Issue number05
DOIs
Publication statusPublished - 2004

Fingerprint Dive into the research topics of 'Three-dimensional ultrastructure of the ring stage of <i>Plasmodium falciparum</i>: Evidence for export pathways'. Together they form a unique fingerprint.

Cite this