Thioquinolobactin, a Pseudomonas siderophore with antifungal and anti-Pythium activity

S Matthijs, K A Tehrani, G Laus, R W Jackson, R M Cooper, P Cornelis

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Abstract

Under conditions of iron limitation Pseudomonas fluorescens ATCC 17400 produces two siderophores, pyoverdine, and a second siderophore quinolobactin, which itself results from the hydrolysis of the unstable molecule 8-hydroxy-4-methoxy-2-quinoline thiocarboxylic acid (thioquinolobactin). Pseudomonas fluorescens ATCC 17400 also displays a strong in vitro antagonism against the Oomycete Pythium, which is repressed by iron, suggesting the involvement of a siderophore(s). While a pyoverdine-negative mutant retains most of its antagonism, a thioquinolobactin-negative mutant only slowed-down Pythium growth, and a double pyoverdine-, thioquinolobactin-negative mutant, which does not produce any siderophore, totally lost its antagonism against Pythium. The siderophore thioquinolobactin could be purified and identified from spent medium and showed anti-Pythium activity, but it was quickly hydrolysed to quinolobactin, which we showed has no antimicrobial activity. Analysis of antagonism-affected transposon mutants revealed that genes involved in haem biosynthesis and sulfur assimilation are important for the production of thioquinolobactin and the expression of antagonism.
Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalEnvironmental Microbiology
Volume9
Issue number2
DOIs
Publication statusPublished - 2007

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    Matthijs, S., Tehrani, K. A., Laus, G., Jackson, R. W., Cooper, R. M., & Cornelis, P. (2007). Thioquinolobactin, a Pseudomonas siderophore with antifungal and anti-Pythium activity. Environmental Microbiology, 9(2), 425-434. https://doi.org/10.1111/j.1462-2920.2006.01154.x