Thermally triggered release of the bacteriophage endolysin CHAPK and the bacteriocin lysostaphin for the control of methicillin resistant Staphylococcus aureus (MRSA)

Hollie Hathaway, Jude Ajuebor, Liam Stephens, Aidan Coffey, Ursula Potter, J. Mark Sutton, A. Toby A Jenkins

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Staphylococcus aureus infections of the skin and soft tissue pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates. As an alternative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess antimicrobial efficacy against multiple species of bacteria including otherwise drug resistant strains. Despite this, the administration and exposure of such antimicrobials should be restricted until required in order to discourage the continued evolution of bacterial resistance, whilst maintaining the activity and stability of such proteinaceous structures. Utilising the increase in skin temperature during infection, the truncated bacteriophage endolysin CHAPK and the staphylococcal bacteriocin lysostaphin have been co-administered in a thermally triggered manner from Poly(N-isopropylacrylamide) (PNIPAM) nanoparticles. The thermoresponsive nature of the PNIPAM polymer has been employed in order to achieve the controlled expulsion of a synergistic enzybiotic cocktail consisting of CHAPK and lysostaphin. The point at which this occurs is modifiable, in this case corresponding to the threshold temperature associated with an infected wound. Consequently, bacterial lysis was observed at 37 °C, whilst growth was maintained at the uninfected skin temperature of 32 °C.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalJournal of Controlled Release
Volume245
DOIs
Publication statusPublished - 10 Jan 2017

Keywords

  • Bacteriocin
  • Bacteriophage endolysin
  • PNIPAM
  • Thermal release

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films

Fingerprint Dive into the research topics of 'Thermally triggered release of the bacteriophage endolysin CHAP<sub>K</sub> and the bacteriocin lysostaphin for the control of methicillin resistant Staphylococcus aureus (MRSA)'. Together they form a unique fingerprint.

Cite this