Projects per year
Abstract
Ensilication is a novel method of protein thermal stabilisation using silica. It uses a modified sol-gel process which tailor fits a protective silica shell around the solvent accessible protein surface. This, electrostatically attached, shell has been found to protect the protein against thermal influences and retains its native structure and function after release. Here, we report the calorimetric analysis of an ensilicated model protein, hen egg-white lysozyme (HEWL) under several ensilication conditions. DSC, TGA-DTA-MS, CD, were used to determine unfolding temperatures of native, released and ensilicated lysozyme to verify the thermal resilience of the ensilicated material. Our findings indicate that ensilication protects against thermal fluctuations even at low concentrations of silica used for ensilication. Secondly, the thermal stabilisation is comparable to lyophilisation, and in some cases is even greater than lyophilisation. Additionally, we performed a mouse in vivo study using lysozyme to demonstrate the antigenic retention over long-term storage. The results suggest that protein is confined within the ensilicated material, and thus is unable to unfold and denature but is still functional after long-term storage.
Original language | English |
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Pages (from-to) | 29789-29796 |
Number of pages | 8 |
Journal | RSC Advances |
Volume | 10 |
Issue number | 50 |
DOIs | |
Publication status | Published - 12 Aug 2020 |
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering
Fingerprint
Dive into the research topics of 'Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis'. Together they form a unique fingerprint.Projects
- 2 Finished
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Role of Rab3 in Peripheral Tissue Insulin Resistance
Koumanov, F. (PI), Betts, J. (CoI), Gonzalez, J. (CoI), Thompson, D. (CoI) & Whitley, P. (CoI)
1/12/16 → 31/07/20
Project: Research council
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Improved Conjugate Vaccines Derived from a Bacterial Immunomodulatory Protein
Van Den Elsen, J. (PI)
Biotechnology and Biological Sciences Research Council
10/10/16 → 9/07/18
Project: Research council
Datasets
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Dataset for "Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis"
Doekhie, A. (Creator), Slade, M. (Creator), Castaing, R. (Creator), Edler, K. (Creator), Koumanov, F. (Creator), Van Den Elsen, J. (Creator), Marchbank, K. (Creator) & Sartbaeva, A. (Creator), University of Bath, 12 Aug 2020
DOI: 10.15125/BATH-00895
Dataset
Equipment
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Differential Scanning Calorimeter (DSC)
Material and Chemical Characterisation (MC2)Facility/equipment: Equipment
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