The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolor

Dimitris Kallifidas, Ben Pascoe, Gillian A Owen, Claire M Strain-Damerell, Hee-Jeon Hong, Mark S B Paget

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Streptomyces coelicolor mutants lacking the zinc-responsive Zur repressor are conditionally defective in sporulation, presumably due to the overexpression of one or more Zur target genes. Gene disruption analyses revealed that deregulation of previously known Zur targets was not responsible for the sporulation phenotype. We used microarrays to identify further Zur targets and discovered that Zur controls a cluster of genes predicted to direct synthesis of an uncharacterized siderophore-related non-ribosomally encoded peptide designated coelibactin. Disruption of a key coelibactin biosynthetic gene suppressed the Zur sporulation phenotype, suggesting that deregulation of coelibactin synthesis inhibits sporulation.

Original languageEnglish
Pages (from-to)608-611
Number of pages4
JournalJournal of Bacteriology
Volume192
Issue number2
DOIs
Publication statusPublished - Jan 2010

Keywords

  • Bacterial Proteins
  • Gene Expression Regulation, Bacterial
  • Multigene Family
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Polymerase Chain Reaction
  • Streptomyces coelicolor

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