The synucleins are a family of redox-active copper binding proteins

Paul Davies, X Y Wang, C J Sarell, A Drewett, Frank Marken, J H Viles, David R Brown

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Thermodynamic studies in conjunction with EPR confirm that alpha-synuclein, beta-synuclein, and gamma-synuclein bind copper(II) in a high affinity 1:1 stoichiometry. gamma-Synuclein demonstrates the highest affinity, in the picomolar range, while alpha-synuclein and beta-synuclein both bind copper(II) with nanomolar affinity. The copper center on all three proteins demonstrates reversible or partly reversible redox cycling. Various mutations show that the primary coordinating ligand for copper(II) is located within the N-terminal regions between residues 2-9. There is also a contribution from the C-terminus in conjunction with the histidine at position 50 in alpha-synuclein and position 65 in beta-synuclein, although these regions appear to have little effect on overall coordination stability. These histidines and the C-terminus, however, appear to be critical to the redox engine of the proteins.
Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalBiochemistry
Volume50
Issue number1
DOIs
Publication statusPublished - 11 Jan 2011

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Synucleins
beta-Synuclein
alpha-Synuclein
Oxidation-Reduction
Copper
gamma-Synuclein
Histidine
Thermodynamics
Stoichiometry
Paramagnetic resonance
Proteins
Ligands
Engines
Mutation
copper-binding protein

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The synucleins are a family of redox-active copper binding proteins. / Davies, Paul; Wang, X Y; Sarell, C J; Drewett, A; Marken, Frank; Viles, J H; Brown, David R.

In: Biochemistry, Vol. 50, No. 1, 11.01.2011, p. 37-47.

Research output: Contribution to journalArticle

Davies, P, Wang, XY, Sarell, CJ, Drewett, A, Marken, F, Viles, JH & Brown, DR 2011, 'The synucleins are a family of redox-active copper binding proteins', Biochemistry, vol. 50, no. 1, pp. 37-47. https://doi.org/10.1021/bi101582p
Davies P, Wang XY, Sarell CJ, Drewett A, Marken F, Viles JH et al. The synucleins are a family of redox-active copper binding proteins. Biochemistry. 2011 Jan 11;50(1):37-47. https://doi.org/10.1021/bi101582p
Davies, Paul ; Wang, X Y ; Sarell, C J ; Drewett, A ; Marken, Frank ; Viles, J H ; Brown, David R. / The synucleins are a family of redox-active copper binding proteins. In: Biochemistry. 2011 ; Vol. 50, No. 1. pp. 37-47.
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