The surface protein Pvs25 of Plasmodium vivax ookinetes interacts with calreticulin on the midgut apical surface of the malaria vector Anopheles albimanus

María del Carmen Rodríguez, Jesús Martínez-Barnetche, Alejandro Alvarado-Delgado, César Batista, Rocío S Argotte-Ramos, Salvador Hernández-Martínez, Lilia González Cerón, Jorge A Torres, Gabriele Margos, Mario Henry Rodríguez

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Malaria parasite transmission-blocking control strategies within the mosquito vector require an adequate understanding of the parasite mosquito interaction at the molecular level. The ookinete P25–P28 surface proteins are required for the transition from ookinete to oocyst in the mosquito midgut; however, their respective molecular interactions in the mosquito are largely unknown. We used recombinant Pvs25 and Pvs28 as probes for identification of potential Anopheles albimanus midgut ligands. A 50 kDa protein interacted with Pvs25 but not with Pvs28 in blot overlay assays. This protein was identified as calreticulin by LS MS and was detected in membrane, but not in soluble midgut protein extracts. Calreticulin was detected in An. albimanus midgut microvilli by immunofluorescence analysis. The An. albimanus calreticulin cDNA was cloned and recombinant calreticulin was shown to interact with recombinant Pvs25 in overlay and co-immunoprecipitation assays, confirming the interaction of the two proteins. The Pvs25-calreticulin interaction in vivo could represent a potential target for developing transmission blocking strategies based on interfering the parasite–midgut interaction.
Original languageEnglish
Pages (from-to)167-177
Number of pages11
JournalMolecular and Biochemical Parasitology
Volume153
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint Dive into the research topics of 'The surface protein Pvs25 of <i>Plasmodium vivax</i> ookinetes interacts with calreticulin on the midgut apical surface of the malaria vector <i>Anopheles albimanus</i>'. Together they form a unique fingerprint.

  • Cite this

    Rodríguez, M. D. C., Martínez-Barnetche, J., Alvarado-Delgado, A., Batista, C., Argotte-Ramos, R. S., Hernández-Martínez, S., González Cerón, L., Torres, J. A., Margos, G., & Rodríguez, M. H. (2007). The surface protein Pvs25 of Plasmodium vivax ookinetes interacts with calreticulin on the midgut apical surface of the malaria vector Anopheles albimanus. Molecular and Biochemical Parasitology, 153(2), 167-177. https://doi.org/10.1016/j.molbiopara.2007.03.002