The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 angstrom. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
|Number of pages||5|
|Journal||Acta Crystallographica Section D-Biological Crystallography|
|Publication status||Published - 2008|
Potter, J. A., Kerou, M., Lamble, H. J., Bull, S. D., Hough, D. W., Danson, M. J., & Taylor, G. L. (2008). The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase. Acta Crystallographica Section D-Biological Crystallography, 64, 1283-1287. https://doi.org/10.1107/S0907444908036111