The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase

J A Potter, M Kerou, H J Lamble, S D Bull, D W Hough, M J Danson, G L Taylor

Research output: Contribution to journalArticle

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Abstract

The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 angstrom. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
Original languageEnglish
Pages (from-to)1283-1287
Number of pages5
JournalActa Crystallographica Section D-Biological Crystallography
Volume64
DOIs
Publication statusPublished - 2008

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Sulfolobus solfataricus
Galactose
Glucose
Sodium Acetate
Archaea
Phosphotransferases
Adenosine Triphosphate
Escherichia coli
2-keto-3-deoxygluconokinase
Enzymes
2-keto-3-deoxygluconate
Proteins

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The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase. / Potter, J A; Kerou, M; Lamble, H J; Bull, S D; Hough, D W; Danson, M J; Taylor, G L.

In: Acta Crystallographica Section D-Biological Crystallography, Vol. 64, 2008, p. 1283-1287.

Research output: Contribution to journalArticle

Potter, J A ; Kerou, M ; Lamble, H J ; Bull, S D ; Hough, D W ; Danson, M J ; Taylor, G L. / The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase. In: Acta Crystallographica Section D-Biological Crystallography. 2008 ; Vol. 64. pp. 1283-1287.
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