The spinning cloth disc reactor for immobilized enzymes

A new process intensification technology for enzymatic reactions

Xudong Feng, Darrell A Patterson, Murat Balaban, Guillaume Fauconnier, Emma A C Emanuelsson

Research output: Contribution to journalArticle

23 Citations (Scopus)
79 Downloads (Pure)

Abstract

The Spinning Cloth Disc Reactor (SCDR) is an innovative enzyme reaction intensification technology. Based on spinning disc technology, the SCDR uses centrifugal forces to allow an even spread of a thin film across a spinning horizontal disc which holds a cloth with immobilized enzyme. This geometry promotes accelerated reactions due to high mass transfer rates and rapid mixing. Here, the SCDR has been benchmarked against a conventional Batch Stirred Tank Reactor (BSTR) using tributyrin emulsion hydrolysis as a model reaction and lipase immobilized on woolen cloth as the biocatalyst. Reaction intensification has been shown to occur: the conversion in the SCDR was significantly higher than that in a conventional BSTR under comparable conditions. Spinning speed and flow rate control reaction rate and conversion: conversion increased nearly 7% on average as the flow rate rose from 2 to 5 mL s-1 and the highest conversion (72.1%) occurred at 400 rpm. A Ping Pong Bi Bi kinetic model fitted reaction progress data well. The immobilized lipase showed excellent stability to repeat reactions in the SCDR: 80% of the original activity was retained after 15 consecutive runs. The robustness of the SCDR to industrially relevant feeds was also demonstrated through successful hydrolysis of different vegetable oils at reaction rates 5 times higher than other reactors in the literature. Overall, the above results indicate that the SCDR is an innovative, superior and robust technology for enhancing enzyme reactions, taking enzyme reactors beyond the current state-of-the-art. This concept can readily be extended to other enzyme-catalyzed reactions, where enhanced mass transfer and enzyme stability is needed.
Original languageEnglish
Pages (from-to)407-417
Number of pages11
JournalChemical Engineering Journal
Volume221
DOIs
Publication statusPublished - 2013

Fingerprint

Immobilized Enzymes
Enzymes
enzyme
Lipases
Lipase
Reaction rates
Hydrolysis
Mass transfer
reaction rate
Flow rate
mass transfer
hydrolysis
reactor
Biocatalysts
Plant Oils
Vegetable oils
Emulsions
vegetable oil
emulsion
Thin films

Cite this

The spinning cloth disc reactor for immobilized enzymes : A new process intensification technology for enzymatic reactions. / Feng, Xudong; Patterson, Darrell A; Balaban, Murat; Fauconnier, Guillaume; Emanuelsson, Emma A C.

In: Chemical Engineering Journal, Vol. 221, 2013, p. 407-417.

Research output: Contribution to journalArticle

Feng, Xudong ; Patterson, Darrell A ; Balaban, Murat ; Fauconnier, Guillaume ; Emanuelsson, Emma A C. / The spinning cloth disc reactor for immobilized enzymes : A new process intensification technology for enzymatic reactions. In: Chemical Engineering Journal. 2013 ; Vol. 221. pp. 407-417.
@article{690dc93aaa8a42be8781c8c39130137e,
title = "The spinning cloth disc reactor for immobilized enzymes: A new process intensification technology for enzymatic reactions",
abstract = "The Spinning Cloth Disc Reactor (SCDR) is an innovative enzyme reaction intensification technology. Based on spinning disc technology, the SCDR uses centrifugal forces to allow an even spread of a thin film across a spinning horizontal disc which holds a cloth with immobilized enzyme. This geometry promotes accelerated reactions due to high mass transfer rates and rapid mixing. Here, the SCDR has been benchmarked against a conventional Batch Stirred Tank Reactor (BSTR) using tributyrin emulsion hydrolysis as a model reaction and lipase immobilized on woolen cloth as the biocatalyst. Reaction intensification has been shown to occur: the conversion in the SCDR was significantly higher than that in a conventional BSTR under comparable conditions. Spinning speed and flow rate control reaction rate and conversion: conversion increased nearly 7{\%} on average as the flow rate rose from 2 to 5 mL s-1 and the highest conversion (72.1{\%}) occurred at 400 rpm. A Ping Pong Bi Bi kinetic model fitted reaction progress data well. The immobilized lipase showed excellent stability to repeat reactions in the SCDR: 80{\%} of the original activity was retained after 15 consecutive runs. The robustness of the SCDR to industrially relevant feeds was also demonstrated through successful hydrolysis of different vegetable oils at reaction rates 5 times higher than other reactors in the literature. Overall, the above results indicate that the SCDR is an innovative, superior and robust technology for enhancing enzyme reactions, taking enzyme reactors beyond the current state-of-the-art. This concept can readily be extended to other enzyme-catalyzed reactions, where enhanced mass transfer and enzyme stability is needed.",
author = "Xudong Feng and Patterson, {Darrell A} and Murat Balaban and Guillaume Fauconnier and Emanuelsson, {Emma A C}",
year = "2013",
doi = "10.1016/j.cej.2013.02.020",
language = "English",
volume = "221",
pages = "407--417",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",

}

TY - JOUR

T1 - The spinning cloth disc reactor for immobilized enzymes

T2 - A new process intensification technology for enzymatic reactions

AU - Feng, Xudong

AU - Patterson, Darrell A

AU - Balaban, Murat

AU - Fauconnier, Guillaume

AU - Emanuelsson, Emma A C

PY - 2013

Y1 - 2013

N2 - The Spinning Cloth Disc Reactor (SCDR) is an innovative enzyme reaction intensification technology. Based on spinning disc technology, the SCDR uses centrifugal forces to allow an even spread of a thin film across a spinning horizontal disc which holds a cloth with immobilized enzyme. This geometry promotes accelerated reactions due to high mass transfer rates and rapid mixing. Here, the SCDR has been benchmarked against a conventional Batch Stirred Tank Reactor (BSTR) using tributyrin emulsion hydrolysis as a model reaction and lipase immobilized on woolen cloth as the biocatalyst. Reaction intensification has been shown to occur: the conversion in the SCDR was significantly higher than that in a conventional BSTR under comparable conditions. Spinning speed and flow rate control reaction rate and conversion: conversion increased nearly 7% on average as the flow rate rose from 2 to 5 mL s-1 and the highest conversion (72.1%) occurred at 400 rpm. A Ping Pong Bi Bi kinetic model fitted reaction progress data well. The immobilized lipase showed excellent stability to repeat reactions in the SCDR: 80% of the original activity was retained after 15 consecutive runs. The robustness of the SCDR to industrially relevant feeds was also demonstrated through successful hydrolysis of different vegetable oils at reaction rates 5 times higher than other reactors in the literature. Overall, the above results indicate that the SCDR is an innovative, superior and robust technology for enhancing enzyme reactions, taking enzyme reactors beyond the current state-of-the-art. This concept can readily be extended to other enzyme-catalyzed reactions, where enhanced mass transfer and enzyme stability is needed.

AB - The Spinning Cloth Disc Reactor (SCDR) is an innovative enzyme reaction intensification technology. Based on spinning disc technology, the SCDR uses centrifugal forces to allow an even spread of a thin film across a spinning horizontal disc which holds a cloth with immobilized enzyme. This geometry promotes accelerated reactions due to high mass transfer rates and rapid mixing. Here, the SCDR has been benchmarked against a conventional Batch Stirred Tank Reactor (BSTR) using tributyrin emulsion hydrolysis as a model reaction and lipase immobilized on woolen cloth as the biocatalyst. Reaction intensification has been shown to occur: the conversion in the SCDR was significantly higher than that in a conventional BSTR under comparable conditions. Spinning speed and flow rate control reaction rate and conversion: conversion increased nearly 7% on average as the flow rate rose from 2 to 5 mL s-1 and the highest conversion (72.1%) occurred at 400 rpm. A Ping Pong Bi Bi kinetic model fitted reaction progress data well. The immobilized lipase showed excellent stability to repeat reactions in the SCDR: 80% of the original activity was retained after 15 consecutive runs. The robustness of the SCDR to industrially relevant feeds was also demonstrated through successful hydrolysis of different vegetable oils at reaction rates 5 times higher than other reactors in the literature. Overall, the above results indicate that the SCDR is an innovative, superior and robust technology for enhancing enzyme reactions, taking enzyme reactors beyond the current state-of-the-art. This concept can readily be extended to other enzyme-catalyzed reactions, where enhanced mass transfer and enzyme stability is needed.

UR - http://www.scopus.com/inward/record.url?scp=84874691680&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1016/j.cej.2013.02.020

U2 - 10.1016/j.cej.2013.02.020

DO - 10.1016/j.cej.2013.02.020

M3 - Article

VL - 221

SP - 407

EP - 417

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

ER -