The secondary structure of influenza A M2 transmembrane domain A circular dichroism study

Kevin C. Duff; Sharon M. Kelly; Nicholas C. Price; Jeremy P. Bradshaw

doi:10.1016/0014-5793(92)81114-2

The secondary structure of influenza A M2 transmembrane domain A circular dichroism study

Kevin C. Duff
, Sharon M. Kelly
, Nicholas C. Price
, Jeremy P. Bradshaw

University of Edinburgh
University of Stirling

Research output: Contribution to journal › Article › peer-review

Abstract

Using circular dichroism, this study investigated the secondary structure of the influenza A M2 transmembrane domain. When reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes, the M2 transmembrane domain was found to adopt a predominantly α-helical secondary structure which was unaffected by both temperature and the addition of 1-aminoadamantane hydrochloride. Reconstitution into 1,2-dioleoyl-sn-glycero-3-phosphoglycerol liposomes resulted in a marked decrease in helical content.

Original language	English
Pages (from-to)	256-258
Number of pages	3
Journal	FEBS Letters
Volume	311
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(92)81114-2
Publication status	Published - 26 Oct 1992

Keywords

Circular dichroism
Influenza M2 protein
Ion channel
Spectrophotometry

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)81114-2

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title = "The secondary structure of influenza A M2 transmembrane domain A circular dichroism study",

abstract = "Using circular dichroism, this study investigated the secondary structure of the influenza A M2 transmembrane domain. When reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes, the M2 transmembrane domain was found to adopt a predominantly α-helical secondary structure which was unaffected by both temperature and the addition of 1-aminoadamantane hydrochloride. Reconstitution into 1,2-dioleoyl-sn-glycero-3-phosphoglycerol liposomes resulted in a marked decrease in helical content.",

keywords = "Circular dichroism, Influenza M2 protein, Ion channel, Spectrophotometry",

author = "Duff, \{Kevin C.\} and Kelly, \{Sharon M.\} and Price, \{Nicholas C.\} and Bradshaw, \{Jeremy P.\}",

year = "1992",

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doi = "10.1016/0014-5793(92)81114-2",

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T1 - The secondary structure of influenza A M2 transmembrane domain A circular dichroism study

AU - Duff, Kevin C.

AU - Kelly, Sharon M.

AU - Price, Nicholas C.

AU - Bradshaw, Jeremy P.

PY - 1992/10/26

Y1 - 1992/10/26

N2 - Using circular dichroism, this study investigated the secondary structure of the influenza A M2 transmembrane domain. When reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes, the M2 transmembrane domain was found to adopt a predominantly α-helical secondary structure which was unaffected by both temperature and the addition of 1-aminoadamantane hydrochloride. Reconstitution into 1,2-dioleoyl-sn-glycero-3-phosphoglycerol liposomes resulted in a marked decrease in helical content.

AB - Using circular dichroism, this study investigated the secondary structure of the influenza A M2 transmembrane domain. When reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes, the M2 transmembrane domain was found to adopt a predominantly α-helical secondary structure which was unaffected by both temperature and the addition of 1-aminoadamantane hydrochloride. Reconstitution into 1,2-dioleoyl-sn-glycero-3-phosphoglycerol liposomes resulted in a marked decrease in helical content.

KW - Circular dichroism

KW - Influenza M2 protein

KW - Ion channel

KW - Spectrophotometry

UR - https://www.scopus.com/pages/publications/0026745087

U2 - 10.1016/0014-5793(92)81114-2

DO - 10.1016/0014-5793(92)81114-2

M3 - Article

C2 - 1397324

AN - SCOPUS:0026745087

SN - 0014-5793

VL - 311

SP - 256

EP - 258

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

The secondary structure of influenza A M2 transmembrane domain A circular dichroism study

Abstract

Keywords

ASJC Scopus subject areas

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