The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts

J L Zhong, G P Edwards, C Raval, H B Li, R M Tyrrell

Research output: Contribution to journalArticle

  • 41 Citations

Abstract

Ultraviolet-A (UVA, 320-380 nm) radiation is an oxidative stress that strongly induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts (FEK4). In this study, we show that NF-E2-related factor 2 (Nrf2) protein accumulates and HO-1 is strongly induced following UVA irradiation of FEK4 cells. Down-regulation of Nrf2 with specific short interfering RNA (siRNA) against Nrf2 (siNrf2) largely abolished the induction of HO-1 following either UVA irradiation or hemin treatment, suggesting that Nrf2 activation mediated modulation of HO-1 by both these agents. Furthermore, a reduction of free heme levels led to a strong decrease in UVA-induced Nrf2 and HO-1 protein levels confirming a clear role for heme in the UV-mediated stress response. Knock-down of Nrf2 protein enhanced membrane damage induced by UVA irradiation, indicating that Nrf2 has a crucial protective role in these cells.
LanguageEnglish
Pages18-24
Number of pages7
JournalPhotochemical & Photobiological Sciences
Volume9
Issue number1
DOIs
StatusPublished - Jan 2010

Fingerprint

NF-E2-Related Factor 2
Heme Oxygenase-1
fibroblasts
Fibroblasts
Skin
induction
Irradiation
proteins
Heme
irradiation
Hemin
Proteins
Oxidative stress
cells
Small Interfering RNA
Chemical activation
Modulation
activation
membranes
damage

Cite this

The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts. / Zhong, J L; Edwards, G P; Raval, C; Li, H B; Tyrrell, R M.

In: Photochemical & Photobiological Sciences, Vol. 9, No. 1, 01.2010, p. 18-24.

Research output: Contribution to journalArticle

@article{a034f66e9d114eab952000786405919e,
title = "The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts",
abstract = "Ultraviolet-A (UVA, 320-380 nm) radiation is an oxidative stress that strongly induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts (FEK4). In this study, we show that NF-E2-related factor 2 (Nrf2) protein accumulates and HO-1 is strongly induced following UVA irradiation of FEK4 cells. Down-regulation of Nrf2 with specific short interfering RNA (siRNA) against Nrf2 (siNrf2) largely abolished the induction of HO-1 following either UVA irradiation or hemin treatment, suggesting that Nrf2 activation mediated modulation of HO-1 by both these agents. Furthermore, a reduction of free heme levels led to a strong decrease in UVA-induced Nrf2 and HO-1 protein levels confirming a clear role for heme in the UV-mediated stress response. Knock-down of Nrf2 protein enhanced membrane damage induced by UVA irradiation, indicating that Nrf2 has a crucial protective role in these cells.",
author = "Zhong, {J L} and Edwards, {G P} and C Raval and Li, {H B} and Tyrrell, {R M}",
year = "2010",
month = "1",
doi = "10.1039/b9pp00068b",
language = "English",
volume = "9",
pages = "18--24",
journal = "Photochemical & Photobiological Sciences",
issn = "1474-905X",
publisher = "Royal Society of Chemistry",
number = "1",

}

TY - JOUR

T1 - The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts

AU - Zhong,J L

AU - Edwards,G P

AU - Raval,C

AU - Li,H B

AU - Tyrrell,R M

PY - 2010/1

Y1 - 2010/1

N2 - Ultraviolet-A (UVA, 320-380 nm) radiation is an oxidative stress that strongly induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts (FEK4). In this study, we show that NF-E2-related factor 2 (Nrf2) protein accumulates and HO-1 is strongly induced following UVA irradiation of FEK4 cells. Down-regulation of Nrf2 with specific short interfering RNA (siRNA) against Nrf2 (siNrf2) largely abolished the induction of HO-1 following either UVA irradiation or hemin treatment, suggesting that Nrf2 activation mediated modulation of HO-1 by both these agents. Furthermore, a reduction of free heme levels led to a strong decrease in UVA-induced Nrf2 and HO-1 protein levels confirming a clear role for heme in the UV-mediated stress response. Knock-down of Nrf2 protein enhanced membrane damage induced by UVA irradiation, indicating that Nrf2 has a crucial protective role in these cells.

AB - Ultraviolet-A (UVA, 320-380 nm) radiation is an oxidative stress that strongly induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts (FEK4). In this study, we show that NF-E2-related factor 2 (Nrf2) protein accumulates and HO-1 is strongly induced following UVA irradiation of FEK4 cells. Down-regulation of Nrf2 with specific short interfering RNA (siRNA) against Nrf2 (siNrf2) largely abolished the induction of HO-1 following either UVA irradiation or hemin treatment, suggesting that Nrf2 activation mediated modulation of HO-1 by both these agents. Furthermore, a reduction of free heme levels led to a strong decrease in UVA-induced Nrf2 and HO-1 protein levels confirming a clear role for heme in the UV-mediated stress response. Knock-down of Nrf2 protein enhanced membrane damage induced by UVA irradiation, indicating that Nrf2 has a crucial protective role in these cells.

UR - http://www.scopus.com/inward/record.url?scp=74049137815&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1039/b9pp00068b

U2 - 10.1039/b9pp00068b

DO - 10.1039/b9pp00068b

M3 - Article

VL - 9

SP - 18

EP - 24

JO - Photochemical & Photobiological Sciences

T2 - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

SN - 1474-905X

IS - 1

ER -